pDUO-hCD14/TLR4A Unit size Cat. code Docs Qty Price
pDUO bearing human CD14/TLR4 Gene
20 µg
pDUO2-mCD14/TLR4 Unit size Cat. code Docs Qty Price
pDUO2 bearing mouse CD14/TLR4 Gene
20 µg

pDUO-CD14/TLR4 is an expression vector designed to co-express CD14 and TLR4 genes known to interact with each other : human CD14 (1125 bp) and human TLR4 (2517 bp) or murine CD14 (480 bp) and murine TLR4 (2505 bp).

The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.

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pDUO plasmid with human CD14 and human TLR4 isoform A genes.
Name: CD14 / TLR4A
Backbone: pDUO
Selection: Blasticidin

pDUO2 plasmid with murine CD14 and murine TLR4 genes.
Name: CD14 / TLR4
Backbone: pDUO2
Selection: Hygromycin

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- 20 µg of lyophilized DNA
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar) or 4 pouches of E. coli Fast-Media® Hygro (2 TB and 2 Agar)

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TLR4 is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to LPS [1]. However, TLR4 alone is not sufficient to confer LPS responsiveness.
TLR4 requires MD-2, a secreted molecule, to functionaly interact with LPS [2]. TLR4 physically associates with MD2, and together with a third protein called CD14, this complex is responsible for LPS recognition and signaling [3].

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282(5396):2085-8.
2. Nagai Y. et al., 2002. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol. 3(7):667-72
3. da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24):21129-35

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pDUO backbone

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