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TRAF6

pUNO1-hTRAF6 Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TRAF6 gene
20 µg
puno1-htraf6
+-
$384.00
pUNO1-hTRAF6-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human TRAF6 gene
20 µg
puno1-htraf6-dn
+-
$406.00
pUNO1-mTRAF6 Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TRAF6 gene
20 µg
puno1-mtraf6
+-
$384.00

Expression-ready ORF Clones

Alias : RNF85

 

TNF receptor associated factor 6.
TNF receptor-associated factor 6, E3 ubiquitin protein ligase.

 

Available versions:

  • Wild type TRAF6
  • Dominant negative TRAF6

Tumor-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) plays a key role in signal transduction of both the TNFR superfamily and the IL-1R/ TLR superfamily. TRAF6 functions as an ubiquitin ligase that mediates the activation of IKK [1]. It participates in both the MyD88-dependent and MyD88-independent signaling pathways induced by TLRs. Upon TLR stimulation TRAF6 interacts with IRAK-1 or TRIF leading to the activation of NF-kB [2,3].

TRAF6-DN

Dominant negative TRAF6 (aa289-522) harbors the C-terminal tumor necrosis factor receptor (TNFR)-associated factor (TRAF) domain but lacks the RING finger domain. Expression of this TRAF6-DN resulted in the suppression of NF-κB activity signalled by interleukin-1 (IL-1) but not by TNF [1].

 

References:

1. Deng L. et al., 2000. Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 103(2):351-61.
2. O’Neill LA., 2002. Signal transduction pathways activated by the IL-1 receptor/toll-like receptor superfamily. Curr Top Microbiol Immunol. 270:47-61.
3. Sato S. et al., 2003. Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-kappaB and IFN-regulatory factor-3, in the Toll-like receptor signaling. J Immunol. 171(8):4304-10.

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Specifications

Human TRAF6 (pUNO1-hTRAF6)

Genbank : NM_145803.2

ORF size : 1569 bp

Subclone : BspHI - NheI

Human TRAF6-DN (pUNO1-hTRAF6-DN)

ORF size : 714 bp

Subclone : NcoI - NheI

Mouse TRAF6 (pUNO1-mTRAF6)

ORF size : 1593 bp

Subclone : BspHI - NheI

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Contents

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

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Citations

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