|pUNO1-hTRAF6||Unit size||Cat. code||Docs||Qty||Price|
pUNO1 bearing the human TRAF6 gene
|pUNO1-hTRAF6-DN||Unit size||Cat. code||Docs||Qty||Price|
pUNO1 bearing a dominant negative version of the human TRAF6 gene
|pUNO1-mTRAF6||Unit size||Cat. code||Docs||Qty||Price|
pUNO1 bearing the mouse TRAF6 gene
Expression-ready ORF Clones
Alias : RNF85
TNF receptor associated factor 6.
TNF receptor-associated factor 6, E3 ubiquitin protein ligase.
- Wild type TRAF6
- Dominant negative TRAF6
Tumor-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) plays a key role in signal transduction of both the TNFR superfamily and the IL-1R/ TLR superfamily. TRAF6 functions as an ubiquitin ligase that mediates the activation of IKK . It participates in both the MyD88-dependent and MyD88-independent signaling pathways induced by TLRs. Upon TLR stimulation TRAF6 interacts with IRAK-1 or TRIF leading to the activation of NF-kB [2,3].
Dominant negative TRAF6 (aa289-522) harbors the C-terminal tumor necrosis factor receptor (TNFR)-associated factor (TRAF) domain but lacks the RING finger domain. Expression of this TRAF6-DN resulted in the suppression of NF-κB activity signalled by interleukin-1 (IL-1) but not by TNF .
1. Deng L. et al., 2000. Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain. Cell 103(2):351-61.
2. O’Neill LA., 2002. Signal transduction pathways activated by the IL-1 receptor/toll-like receptor superfamily. Curr Top Microbiol Immunol. 270:47-61.
3. Sato S. et al., 2003. Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-kappaB and IFN-regulatory factor-3, in the Toll-like receptor signaling. J Immunol. 171(8):4304-10.
Human TRAF6 (pUNO1-hTRAF6)
Genbank : NM_145803.2
ORF size : 1569 bp
Subclone : BspHI - NheI
Human TRAF6-DN (pUNO1-hTRAF6-DN)
ORF size : 714 bp
Subclone : NcoI - NheI
Mouse TRAF6 (pUNO1-mTRAF6)
Genbank : NM_009424.3
ORF size : 1593 bp
Subclone : BspHI - NheIBack to the top
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.Back to the top