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TIRAP

pUNO1-hTIRAPb Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TIRAP gene, isoform 2 (longest)
20 µg
puno1-htirap
+-
$384.00
pUNO1-hTIRAPb-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human TIRAP gene
20 µg
puno1-htirap-dn
+-
$406.00
pUNO1-mTIRAP Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TIRAP gene
20 µg
puno1-mtirap
+-
$384.00

Expression-ready ORF Clones

Alias : MAL, wyatt

 

TIR domain containing adaptor protein.
MyD88 adapter-like.

 

Available versions:

  • Wild type TIRAP
  • Dominant negative TIRAP

TIR domain containing adaptor protein (TIRAP), also known as Mal, is differentially involved in the signaling to NF-kB by members of the TLR family. TIRAP-deficient mice were shown to respond normally to the TLR3, TLR5, TLR7 and TLR9 ligands, but to be defective in cytokine response to LPS, a ligand for TLR4, as well as ligands for TLR2, TLR1 and TLR6 [1,2]. TIRAP contributes to TLR2 and TLR4 MyD88-dependent signaling pathway but interacts only with TLR4 to activate a MyD88-independent pathway to induce IFN-β and STAT-1 dependent genes [3].

TIRAP-DN

Dominant negative TIRAP (P125H) was generated by mutating the proline at position 125 into a histidine. This TIRAP-DN was shown to inhibit NF-κB activated by LPS-induced TLR4 but not NF-κB activated by IL-1RI or IL-18R suggesting that TIRAP is involved in TLR4 signaling [4].

1. Horng T. et al. 2002. The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors. Nature 420(6913):329-33
2. Yamamoto M. et al. 2002. Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4. Nature 420(6913):324-9
3. Toshchakov V. et al., 2003. TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses. J Endotoxin Res. 9(3):169-75.
4. Fitzgerald KA et al., 2001. Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction. Nature. 2001 Sep 6;413(6851):78-83.

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Specifications

Human TIRAP isoform 2 (longest) (pUNO1-hTIRAPb)

Genbank : NM_148910.2 with silent variation in codon Ala186
ORF size : 708 bp
Subclone : NcoI - NheI

Human TIRAP-DN (pUNO1-hTIRAPb-DN)

ORF size : 708 bp
Subclone : NcoI - NheI

Mouse TIRAP (pUNO1-mTIRAP)

Genbank : NM_001177845.1 with silent variation in codon Pro70
ORF size : 726 bp
Subclone : NcoI - NheI

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Contents

- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml

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