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TRIF (TICAM1)

pUNO2-hTRIF Unit size Cat. code Docs Qty Price
pUNO2 bearing the human TRIF gene
20 µg
puno2-htrif
+-
$384.00
pUNO1-hTRIF-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human TRIF gene
20 µg
puno1-htrif-dn
+-
$406.00
pUNO1-hsaTRIFΔ Unit size Cat. code Docs Qty Price
pUNO1 bearing the human saTRIFΔ (super-activated TRIF) gene
20 µg
puno1-hsatrifd
+-
$384.00
pUNO1-mTRIF Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TRIF gene
20 µg
puno1-mtrif
+-
$384.00

Expression-ready ORF Clones

Alias : TICAM1

 

Toll like receptor adaptor molecule 1.
TIR domain-containing adapter inducing IFNbeta.
TIR-containing adaptor molecule 1.

 

Available versions:

  • Wild type TRIF
  • Dominant negative TRIF

TRIF (TICAM1) is an adapter involved in the MyD88-independent signaling pathway of several TLRs. A dominant-negative form of TRIF was shown to completely abolish TLR3-induced signaling and also to block the NF-kB activity mediated by TLR2, TLR4 and TLR7 [1]. Further analysis has revealed that TRIF binds preferentially to TLR3 and TLR4 and is essential in their signaling pathways [2]. It predominantly activates IRF-3 and the production of IFN-b rather than NF-kB [3].

TRIF-DN

Dominant negative TRIF (aa387-566) harbors only the TIR domain and lacks the N-terminal and C-terminal portions [1]. Furthermore, it contains a proline to histidine mutation at amino acid 434. This proline residue is conserved among TLRs and is essential for activation of the TLR-mediated signaling.

1. Yamamoto M. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-b promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672
2. Yamamoto M. et al., 2003. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science. 301(5633):640-3.
3. Oshiumi H. et al., 2003. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.Nat Immunol. 4(2):161-7.

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Specifications

Human TRIF (pUNO2-hTRIF)

ORF size : 2139 bp
Subclone : BspHI - NheI

Human TRIF-DN (pUNO1-hTRIF-DN)

ORF size : 546 bp
Subclone : NcoI - NheI

Human saTRIFΔ (pUNO1-hsaTRIFΔ)

ORF size : 1935 bp
Subclone : BspHI - NheI

Mouse TRIF (pUNO1-mTRIF)

Genbank : NM_174989.4
ORF size : 2199 bp
Subclone : AgeI - EcoRI

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Contents

pUNO1:
- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml

pUNO2 :
- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media®  Zeo (2 TB and 2 Agar)
- 1 ml Zeocin™ at 100 mg/ml

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Citations

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