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TRAM (TICAM2)

pUNO1-hTRAM Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TRAM (TICAM2) gene
20 µg
puno1-htram
+-
$384.00
pUNO1-hTRAM-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human TRAM (TICAM2) gene
20 µg
puno1-htram-dn
+-
$406.00
pUNO1-mTRAM Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TRAM (TICAM2) gene
20 µg
puno1-mtram
+-
$384.00

Expression-ready ORF Clones

Alias : TICAM2, TIRP

 

TRIF-related adapter molecule.
Toll like receptor adaptor molecule 2.

 

Available versions:

  • Wild type TRAM
  • Dominant negative TRAM

TRIF-related adaptor molecule (TRAM), also known as TIR-containing adaptor molecule (TICAM)-2, is a recently characterized adaptor molecule involved in the MyD88-independent TLR4 signaling pathway [1]. In response to LPS stimulation, TRAM physically bridges TLR4 and TRIF transmitting LPS-induced signaling to TRIF which in turn activates IRF-3 and the production of interferon beta [2]. TRAM function is restricted to the TLR4 pathway, as TRAM-deficient mice are defective in cytokine production in response to TLR4 ligand but not to other TLR ligands [3].

TRAM-DN

Dominant negative TRAM/TICAM2 (C117H) was generated by mutating the cysteine at position 117 into a histidine. This TRAM-DN was shown to retain its ability to bind to TLR4 but to lose its homo (TICAM2-TICAM2) and hetero (TICAM1-TICAM2) dimerizing properties [2].

1. Fitzgerald KA. et al., 2003. LPS-TLR4 Signaling to IRF-3/7 and NF-{kappa}B Involves the Toll Adapters TRAM and TRIF. J Exp Med. 198(7):1043-1055
2. Oshiumi H. et al., 2003. TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta. J Biol Chem. 278(50):49751-62
3. Yamamoto M. et al., 2003. TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway. Nat Immunol. 4(11):1144-50

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Specifications

Human TRAM (pUNO1-hTRAM)

Genbank : NM_021649.7
ORF size : 708 bp
Subclone : NcoI - NheI

Human TRAM-DN (pUNO1-hTRAM-DN)

ORF size : 708 bp
Subclone : NcoI - NheI

Mouse TRAM (pUNO1-mTRAM)

Genbank : NM_173394.3
ORF size : 699 bp
Subclone : NcoI - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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