NLRC4 Reporter Assay

InvivoGen has developed a new cell-based assay to monitor the activation of the NLRC4 inflammasome by intracellular flagellin.

This assay relies on two cell lines, an NLRC4 inflammasome test cell line, THP1-NLRC4, and a TLR5-deficient IL-1β reporter cell line, HEK-Blue™ KD-TLR5, which can be used sequentially or co-cultured to save time.

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