2’3’-c-di-AM(PS)2 (Rp,Rp)

2’3’-c-di-AM(PS)2 (Rp,Rp) Unit size Cat. code Docs Qty Price
Bisphosphorothioate analog of c-di-AMP, Rp isomers
100 µg
500 µg

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Bisphosphorothioate analog of 2'3'-c-di-AMP, (Rp;Rp) cyclic [A(2’,5’)psA(3’,5’)ps]

2’3’-c-di-AM(PS)2 (Rp,Rp) is the Rp,Rp-isomer of the 2’3’ bisphosphorothioate analog of 3’3’-cyclic adenosine monophosphate (c-di-AMP). c-di-AMP is second messenger molecule produced by bacteria that has potent immunostimulant activity in mammals [1].

This cyclic dinucleotide (CDN) induces the production of type I interferons (IFNs) following its recognition by the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes) and the recruitment of TBK1 (TANK-binding kinase 1) and IRF3 (interferon regulatory factor 3) [2].

2’3’-c-di-AM(PS)(Rp,Rp) has a higher affinity for STING than c-di-AMP due to the presence of a 2’-5’, 3’-5’ mixed linkage, as found in endogenous human CDNs produced by cGAS (cyclic GMP-AMP (cGAMP) synthase) [3]. It activates all known human STING alleles as well as mouse STING.

In addition, this analog contains two phosphorothioate diester linkages to protect it against degradation by phosphodiesterases that are present in host cells or in the systemic circulation [4].

The Rp, Rp dithio diastereoisomer was found to induce higher type I IFN production compared to the Rp/Sp dithio diastereoisomers or c-di-AMP [3]In vivo studies demonstrated that 2’3’-c-di-AM(PS)2 (Rp,Rp) could potently prime tumor antigen–specific CD8+ T-cell responses and could overcome antigen-enforced immune tolerance in combination with PD-L1 blockade [5]

InvivoGen has developed STING reporter cells in two well established immune cell models: human THP-1 monocytes and mouse RAW 264.7 macrophages.

These cells express a reporter gene (SEAP or Lucia luciferase) under control of an IRF-inducible and/or NF-κB-inducible promoter.



1. Woodward JJ. et al., 2010. c-di-AMP secreted by intracellular Listeria monocytogenes activates a host type I interferon response. Science.328(5986):1703-5.
2. Jin L. et al., 2011. MPYS is required for IFN response factor 3 activation and type I IFN production in the response of cultured phagocytes to bacterial second messengers cyclic-di-AMP and cyclic-di- GMP. J Immunol. 187(5):2595-601.
3. Corrales L et al., 2015. Direct activation of STING in the tumor microenvironment leads to potent and systemic tumor regression and immunity. Cell Rep. 11(7):1018-30.
4. Yan H. et al., 2008. Synthesis and immunostimulatory properties of the phosphorothioate analogues of cdiGMP. Bioorg. Med. Chem. Lett. 18, 5631–5634.
5. Foote JB et al., 2017. A STING Agonist Given with OX40 Receptor and PD-L1 Modulators Primes Immunity and Reduces Tumor Growth in Tolerized Mice. Cancer Immunol Res. 5(6):468-479.


IRF INDUCTION (Lucia luciferase reporter)
IRF INDUCTION (Lucia luciferase reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). IRF induction was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI‑Luc™, a Lucia luciferase detection reagent.

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). NF‑kB induction was determined using QUANTI‑Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) have been included as a negative control.

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Synonyms: (R,R)-(2',3')c-diAM(PS)2, (2',3')-Rp,Rp-c-diAMPSS

Formula: C20H22N10O10P2S2 .2Na

Molecular weight: 734.50

Purity: ≥ 95% by LC/MS & NMR

Solubility: 50 mg/ml in water

CAS number: 1638750-95-4

Quality control:

  • The ability of 2’3’-c-di-AM(PS)2 (Rp,Rp) to induce type I interferon (IFN) has been confirmed in THP1-Blue™ ISG cells.
  • The absence of bacterial contamination (lipoproteins & endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
  • Purity and structure controlled by LC/MS and NMR: ≥ 95%.
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2’3’-c-di-AM(PS)2 (Rp,Rp) is provided as a lyophilized powder and is available in two quantities:

  • tlrl-nacda2r-01: 100 μg 2’3’-c-di-AM(PS)2 (Rp,Rp) + 1.5 ml endotoxin-free water.
  • tlrl-nacda2r: 500 μg 2’3’-c-di-AM(PS)2 (Rp,Rp) + 1.5 ml endotoxin-free water.

Note: 2’3’-c-di-AM(PS)(Rp,Rp) is sterile filtered prior to lyophilization.

2’3’-c-di-AM(PS)2 (Rp,Rp) is shipped at room temperature

2’3’-c-di-AM(PS)2 (Rp,Rp) should be stored at -20°C.

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Structure of 23-cdiAMPS2


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