|Anti-hTNF-α-hIgG4||Unit size||Cat. code||Docs||Qty||Price|
Human TNF-α (adalimumab) antibody - Human IgG4
Neutralizing human IgG4 monoclonal antibody against human TNF-α
Anti-hTNF-α-hIgG4 is a neutralizing monoclonal antibody featuring the constant region of the human IgG4 isotype and the variable region of adalimumab. Adalimumab is a fully human monoclonal antibody against the pro-inflammatory cytokine human tumor necrosis factor alpha (hTNF-α).
Adalimumab binds to TNF-α and blocks its interaction with TNF receptors thereby downregulating the inflammatory reactions associated with autoimmune diseases, such as rheumatoid arthritis and Crohn’s disease.
Anti-hTNF-α-hIgG4 was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography with protein G.
The neutralizing activity of this human IgG4 antibody was determined using HEK-Blue™ TNF-α/IL-1β cells.Back to the top
ND50 (50% neutralizing dose): ~ 2 - 5 ng/ml for 4 ng/ml human TNF-α in HEK-Blue™ TNF-α/IL-1β cells
Clonality: Monoclonal antibody
Application: Neutralization of human TNF-α-induced cellular activation
Specificity: Human TNF-α
Isotype: Human IgG4
Formulation: 0.2 µm filtered solution in 75 mM phosphate buffer with 100 mM glycine, 5% w/v saccharose and stabilizing agentsBack to the top
- 100 µg purified anti-hTNFα-hIgG4 antibody, provided azide-free and lyophilized
Product is shipped at room temperature.
Store lyophilized antibody at -20 °C.
Lyophilized product is stable for at least 1 yearBack to the top