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Anti-hTNF-α-hIgG4

Anti-hTNF-α-hIgG4 Unit size Cat. code Docs Qty Price
Human TNF-α (adalimumab) antibody - Human IgG4
100 µg
htnfa-mab4
+-
$315.00

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Neutralizing human IgG4 monoclonal antibody against human TNF-α

Anti-hTNF-α-hIgG4 is a neutralizing monoclonal antibody (mAb) featuring the constant region of the human IgG4 (hIgG4) isotype and the variable region of adalimumab, a fully human therapeutic mAb that targets human tumor necrosis factor-alpha (hTNF-α). TNF-α is a pleiotropic cytokine that notably induces NF-κB-mediated production of pro-inflammatory cytokines [1,2]. The variable region of adalimumab blocks the interaction of TNF-α with its receptors TFNR1 and TNFR2, thereby downregulating the inflammatory responses associated with autoimmune diseases, such as rheumatoid arthritis and Crohn’s disease [1,2]. The hIgG4 isotype displays low to no potency for Fc-mediated antibody-dependent cellular cytotoxicity (ADCC), low potency for antibody-dependent cellular phagocytosis (ADCP), and it does not mediate complement-dependent cytotoxicity (CDC). 

Anti-hTNF-α-hIgG4 was generated by recombinant DNA technology. It is produced in CHO cells and purified by affinity chromatography with protein G. The neutralizing activity of anti-hTNF-α-hIgG4 antibody was determined using HEK-Blue™ TNF-α cells.

 

Review Learn more on antibody isotype effector functions by reading our flyer on Clinically-relevant monoclonal antibodies

 

References:

1. Steeland S., et al. 2018. A new venue of TNF targeting. Int. J. Mol. Sci. 19:1442.
2. Shealy D.J. & Visvanathan S. 2008. Anti-TNF antibodies: lessons from the past, roadmap for the future. Therapeutic Antibodies (book). 101-129.

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Specifications

ND50 (50% neutralizing dose):  ~ 2 - 5 ng/ml  for 4 ng/ml human TNF-α in HEK-Blue™ TNF-α cells

Specificity: Human TNF-α (hTNF-α)
Clonality: Monoclonal antibody
Isotype: Human IgG4
Source: Chinese hamster ovary (CHO) cells
Purification: Affinity chromatography with protein G
Formulation: 0.2 µm filtered solution in sodium phosphate buffer with glycine, saccharose and stabilizing agents
Tested application: Neutralization of hTNF-α signaling.

Quality control:

  • Human IgG4 isotype has been confirmed by ELISA.
  • The neutralization of hTNF-α signaling pathway has been confirmed using cellular assays with the HEK-Blue™ TNF-α cells.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
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Contents

  • 100 µg purified anti-hTNFα-hIgG4 antibody, provided azide-free and lyophilized

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stability Lyophilized product is stable for at 1 year when stored at -20°C.

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