|Anti-hIL-18-IgG||Unit size||Cat. code||Docs||Price|
Neutralizing hIgG1 monoclonal antibody to human interleukin 18
3 x 100 µg
Neutralizing IgG monoclonal antibody to human interleukin 18
Anti-hIL-18-IgG is a monoclonal antibody specific for human interleukin 18 (hIL-18). It has been selected for its ability to efficiently neutralize the biological activity of hIL-18. Anti-hIL-18-IgG was generated by recombinant DNA technology.
Interleukin 18 (IL-18; formerly called interferon-γ inducing factor) is a pro-inflammatory cytokine that causes a wide variety of biological effects associated with infection, inflammation and autoimmune processes [1, 2].
More specifically, IL-18 induces IFN-γ production and contributes to T-helper 1 (Th1) cell polarization. IL-18 is produced by macrophages and other cells, as a proprotein which is proteolytically processed to its active form by caspase 1, an enzyme that is activated within the inflammasome multiprotein complex. IL-18 binds to a heterodimeric receptor consisting of IL-18R and IL-18 receptor accessory protein (IL-18RAP). Upon binding, IL-18 activates NF-κB and AP-1 via signaling pathways that involve IRAK and TRAF-6 [3, 4].
Anti-hIL-18-IgG has been produced in Chinese hamster ovarian (CHO) cells and purified by affinity chromatography.
1. Arend W. et al., 2008. IL-1, IL-18, and IL-33 families of cytokines. Immunol Rev. 223:20-38.
2. Dinarello CA. et al., 1998. Overview of interleukin-18: more than an interferon-gamma inducing factor. J Leukoc Biol. 63(6):658-64.
3. Gracie JA. et al., 2003. Interleukin-18. J Leukoc Biol.3(2):213-24.
4. Kojima H. et al., 1998. Interleukin-18 activates the IRAK-TRAF6 pathway in mouse EL-4 cells. BBRC 244(1):183-6.
Applications: Anti-hIL-18-IgG is a neutralizing antibody, it blocks hIL-18-induced cellular activation.
Target: Natural and recombinant human interleukin 18 (hIL-18).
Clonality: Monoclonal antibody
Isotype: Human IgG1, kappa
Source: CHO cells
Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine and stabilizing agents
Purity: Purified by affinity chromatography with protein G
3 x 100 μg purified anti-hIL18-IgG antibody, provided azide-free and lyophilized
Product is shipped at room temperature.
Store lyophilized antibody at -20 °C.