293/TLR Cells

HEK 293 cells expressing one or two TLR and/or TLR-related genes

293/TLR cells are HEK293 cells stably transfected with a plasmid that constitutively expresses one or two TLR and/or TLR-related genes, of human or mouse origin.

They can be used to determine TLR activation upon ligand stimulation by assessing IL-8 production or NF-κB activation.

The latter can be evaluated by transfecting transiently or stably 293/TLR cells with pNiFty, a family of NF-κB inducible reporter plasmids expressing either the secreted alkaline phosphatase or luciferase reporter gene.