293/TLR cells

293/TLR cells are HEK293 cells stably transfected with a plasmid that constitutively expresses one or two TLR and/or TLR-related genes, of human or mouse origin. They can be used to determine TLR activation upon ligand stimulation by assessing IL-8 production or NF-κB activation. The latter can be evaluated by transfecting transiently or stably 293/TLR cells with pNiFty, a family of NF-κB inducible reporter plasmids expressing either the secreted alkaline phosphatase or luciferase reporter gene.