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pDRIVE-AFP

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-hAFP

pDRIVE bearing human AFP promoter - LacZ

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20 µg

pdrive-hafp
+-
$554

pDRIVE5SEAP-hAFP

pDRIVE bearing human AFP promoter - SEAP

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20 µg

pdrive5s-hafp
+-
$554

pDRIVE5Lucia-hAFP

pDRIVE bearing human AFP promoter - Lucia

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20 µg

pdrive5lc-hafp
+-
$554
  • About
  • Specifications
  • pDRIVE contents
  • Details

AFP promoter (Hepatocellular carcinoma) in pDRIVE expression plasmid

The alpha-fetoprotein (AFP) gene is normally expressed in fetal but not adult livers. However, about 70% of hepatocellular carcinoma (HCC) are known to overexpress AFP, this up-regulation occuring at the transcriptional level.

The AFP promoter has been extensively studied and shown to confer a selective expression of a transgene in vitro and in vivo [1].

The expression level of the transgene is proportional to the level of AFP expression in the transfected cells [2].

Several studies have reported the use of AFP promoter to express a cytokine or suicide gene into HCC cells after delivery with viral or plasmid vectors [1, 2]. 

 

References:

1. Kanai F. et al. 1997. In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene. Cancer Res. 57(3):461-5.
2. He P. et al. 2000. The targeted expression of interleukin-2 in human hepatocellular carcinoma cells. J Exp Clin Cancer Res. 19(2):183-7.

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Specifications

pDRIVE bearing human AFP promoter - LacZ, SEAP or Lucia

Gene Promoter: Alpha-Fetoprotein

Specificity: Hepatocellular carcinoma

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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