dsDNA-EC
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Cat.code:
tlrl-ecdna
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ABOUT
Double-stranded genomic DNA from E. coli K12
dsDNA-EC is an ultrapure preparation of double-stranded DNA from Escherichia coli K12.
Transfected dsDNA-EC is recognized by cytosolic DNA sensors (CDSs) such as cyclic GMP-AMP synthase (cGAS, cGAMP synthase) leading to the production of type I interferons through the STING-TBK1-IRF3 pathway. It is also recognized by the endoplasmic receptor Toll-like receptor 9 (TLR9).
Recognition of dsDNA-EC by CDSs can be studied in THP1-derived reporter cells such as THP1-Blue™ ISG cells, an IRF-SEAP reporter cell line. Recognition of dsDNA-EC by TLR9 can be studied in HEK-Blue™ TLR9 cells, which are HEK293-derived cells that stably express TLR9 and an NF-κB-SEAP reporter construct.
To facilitate the intracellular delivery, complex dsDNA-EC with a cationic lipid transfection agent, such as LyoVec™.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
TLR9, CDS, cGAS
30 ng - 1 μg/ml
1 mg/ml in water
< 0.01 EU/µg (measurement by kinetic chromogenic LAL assay)
Cellular assays
TLR9 activation
cGAS activation
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:dsDNA-EC
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Cat code:tlrl-ecdna
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Quantity:200 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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