pDRIVE-ICAM-2

ICAM-2 promoter (Endothelial cells) in pDRIVE expression plasmid

ICAM-2 is a cell surface adhesion molecule constitutively expressed in vasculature endothelial cells and megakaryocytes.

Endothelial-specific activity of the ICAM-2 promoter resides in a 334 bp fragment in the 5’ flanking region of the gene [1].

The promoter contains no TATA box but four transcription factor binding sites such as Sp1 and GATA motifs, essential for basal and endothelial-specific expression of the ICAM-2 gene.

The ICAM-2 promoter has been used to generate transgenic animals which demonstrated endothelial expression of a transgene [2,3].

References:

1. Cowan PJ. et al., 1998. The human ICAM-2 promoter is endothelial cell-specific in vitro and in vivo and contains critical Sp1 and GATA binding sites. J Biol Chem. 273(19):11737-44.
2. Cowan PJ. et al., 1996. Targeting gene expression to endothelial cells in transgenic mice using the human intercellular adhesion molecule 2 promoter. Transplantation. 62(2):155-60.
3. Velasco B. et al.,. 2001. Vascular gene transfer driven by endoglin and ICAM-2 endothelial-specific promoters. Gene Ther. 8(12):897-904.