Invivogen
Menu

pDRIVE-OG-2

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-mOG2

pDRIVE bearing mouse OG-2 promoter - LacZ

Show product

20 µg

pdrive-mog2
+-
$554

pDRIVE5SEAP-mOG2

pDRIVE bearing mouse OG-2 promoter - SEAP

Show product

20 µg

pdrive5s-mog2
+-
$554

pDRIVE5Lucia-mOG2

pDRIVE bearing mouse OG-2 promoter - Lucia

Show product

20 µg

pdrive5lc-mog2
+-
$554

OG-2 promoter (Osteoblasts, Odonblasts) in pDRIVE expression plasmid

Osteocalcin gene 2 (OG2) expression is restricted to mature osteoblasts and odontoblasts and is regulated at the transcriptional level by hormones like Vit D3 and growth factors like TNFα.

The OG2 proximal promoter located between -147 and -34 is sufficient to confer osteoblast-specific expression to a reporter gene [1].

This region contains two osteoblast-specific cis-acting elements called OSE1 and OSE2 [2]. Multimers of each of these elements confer osteoblast specific activity to a heterologous promoter.

Addition of other regulatory elements located between 647 and 147 can further increase the expression level of a transgene in vivo [3].

 

References:

1. Ducy P. & Karsenty G. 1995. Two distinct osteoblast-specific cis-acting elements control expression of a mouse osteocalcin gene. Mol Cell Biol 15(4):1858-69.
2. Schinke T. & Karsenty G. 1999. Characterization of Osf1, an osteoblast-specific transcription factor binding to a critical cis-acting element in the mouse Osteocalcin promoters. J Biol Chem. 274(42):30182-9.
3. Frendo JL. et al. 1998. Functional hierarchy between two OSE2 elements in the control of osteocalcin gene expression in vivo. J Biol Chem 273(46):30509-16.

Back to the top

Specifications

pDRIVE bearing mouse OG-2 promoter - LacZ, SEAP or Lucia

Gene Promoter: Osteocalcin-2

Specificity: Osteoblasts, Odonblasts

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

Back to the top

pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

Back to the top

Details

pDRIVE map

pDRIVE5-SEAP map

Back to the top
Customer Service
& Technical Support
Shopping cart is empty