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pUNO1 bearing the Goldenticket (Gt) isoform mouse STING (I199N) gene
STING (stimulator of interferon genes; also known as TMEM173, MITA, MPYS, and ERIS) is essential for the IFN response to microbial or self-DNA, and acts as a direct sensor of cyclic dinucleotides (CDNs).
CDNs are important messengers in bacteria, affecting numerous responses of the prokaryotic cell, but also in mammalian cells, acting as agonists of the innate immune response.
Studies have revealed that STING variation can affect CDN recognition and signal transduction.
The I199N mutation carried by the Goldenticket (Gt) mouse strain fails to produce type I IFNs upon bacterial infection or in response to c-di-GMP, a bacterial CDN1. The I199N missense mutation lies in exon 6 of the mouse STING gene and results in a null-phenotype with no detectable STING activity.
1. Sauer JD. et al., 2011. The N-ethyl-N-nitrosourea-induced Goldenticket mouse mutant reveals an essential function of Sting in the in vivo interferon response to Listeria monocytogenes and cyclic dinucleotides. Infect Immun 79(2):688-94.
Human STING-Gt (pUNO1-mSTING-Gt)
ORF size : 1137 bp
Subclone : AgeI - NheI