HKEB Unit size Cat. code Docs Qty Price
Heat Killed E.coli 0111:B4
10e10 cells

Heat Killed E. coli 0111:B4

HKEB is a heat killed preparation of the gram negative bacterium, E.coli O111:B4. Cell wall components from this bacterium, such as peptidoglycan (PGN) and lipopolysaccharide (LPS), are recognized by the toll-like receptor (TLR) 2 and TLR4 [1].

It has been demonstrated that HKEB can stimulate TLR2 and induce the production of NF-κB and pro-inflammatory cytokines, such as IL-8 [2]. HKEB is a potent stimulator of TLR2 and TLR4.



1. Takeuchi O. et al., 1999. Differential roles of TLR2 and TLR4 in recognition of gram negative and gram-positive bacterial cell wall components. Immunity, 11(4):443-51.
2. van Riet E. et al., 2009. Combined TLR2 and TLR4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for TH1/TH2 polarization. BMC Immunology, 10:9.

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Specificity: TLR2 / TLR 4 agonist

Working concentration: 105-107 cells/ml

Endotoxin level: >1 EU/109 cells

Solubility: Insoluble (resuspended HKEB results in a cloudy suspension)

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HKEB is provided lyophilized:

  • 1010 freeze-dried cells of Heat Killed E.coli 0111:B4 (HKEB)
  • 1.5 ml sterile endotoxin-free water

HKEB is shipped at room temperature.

Store at 4°C. Upon resuspension, prepare aliquots of HKEB and store at 4°C for short term storage or -20°C for long storage.

Resuspended product is stable 1 month at 4°C and at least 1 year at -20°C when properly stored.

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