HKEB - TLR2 Agonist

Heat Killed - E.coli 0111:B4

ABOUT

Heat Killed E. coli 0111:B4

HKEB is a heat killed preparation of the gram negative bacterium, E.coli O111:B4. Cell wall components from this bacterium, such as peptidoglycan (PGN) and lipopolysaccharide (LPS), are recognized by the toll-like receptor (TLR) 2 and TLR4 [1].

It has been demonstrated that HKEB can stimulate TLR2 and induce the production of NF-κB and pro-inflammatory cytokines, such as IL-8 [2]. HKEB is a potent stimulator of TLR2 and TLR4. 

 

References:

1. Takeuchi O. et al., 1999. Differential roles of TLR2 and TLR4 in recognition of gram negative and gram-positive bacterial cell wall components. Immunity, 11(4):443-51.
2. van Riet E. et al., 2009. Combined TLR2 and TLR4 ligation in the context of bacterial or helminth extracts in human monocyte derived dendritic cells: molecular correlates for TH1/TH2 polarization. BMC Immunology, 10:9.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
E. coli O111:B4
Working concentration

105-107 cells/ml

Solubility

Insoluble (resuspended HKEB results in a cloudy suspension)

Endotoxin

>1 EU/109 cells

Tested applications

Cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    HKEB
  • Cat code: 
    tlrl-hkeb2
  • Quantity: 
    10^10 cells
Includes:

1.5 ml sterile endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended product is stable 1 month at 4°C and at least 1 year at -20°C when properly stored.

DOCUMENTS

Documents

HKEB

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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