ODN D-SL03 - TLR9 Agonist

Class C CpG oligonucleotide - Multispecies TLR9 ligand

ODN D-SL03 (double-stem loop ODN) is a synthetic oligonucleotide that contains unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs), that are recognized by TLR9 leading to strong immunostimulatory effects.

ODN D-SL03 is a type C CpG ODN and a TLR9 agonist in a diverse vertebrate species, namely humans, mice, rats, rabbits, pigs and dogs.

ODN D-SL03 is composed of double stem loops, a phosphorothioate backbone and two palindromes with AACGTT motif and TTCGAA motif in each loop.

ODN D-SL03 is a robust inducer of IFN-α apparently due to the presence of the palindrome sequence. D-SL03 has been shown to potently activate human B cells, NK cells and mononuclear cells as well as PBMC/splenocytes obtained from diverse vertebrate species, namely mice, rats, rabbits, dogs and pigs.

D-SL03 activates B cells and NK cells. ODN D-SL03 demonstrates anti-tumor activity in mice with established breast cancer [1].

Reference:

1. Yang L. et al., 2013. CpG oligodeoxynucleotides with double stem-loops show strong immunostimulatory activity. Int Immunopharmacol. 15(1):89-96.