NF-κB Dual Reporter HEK 293 Cells - HEK-Blue-Lucia™ Null Cells

NF-κB-SEAP & -Lucia Reporter Cells

ABOUT

Double NF-κB–readout HEK293 reporter cells

InvivoGen offers a human embryonic kidney 293 (HEK293)-derived cell line, specifically designed to assess the distinct role of various Toll-like receptors (TLRs):

— HEK-Blue-Lucia™ Null cells*

These cells express two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia® luciferase. Stimulation of HEK-Blue-Lucia™ Null cells with TLR agonists triggers the activation of the artificial NF-κB-inducible promoter and the subsequent production of SEAP. It also promotes the expression of Lucia® luciferase, which is knocked in (KI) downstream of the endogenous (more physiological) IL-8 promoter. IL-8 (interleukin 8) is a chemokine produced in response to TLR agonists in an NF-κB/AP-1-dependent manner [1-2].


This feature enables the double readout study of the NF-κB/AP-1 pathway, by monitoring the activity of SEAP and Lucia® luciferase using QUANTI-Blue™ Solution (SEAP detection reagent) or QUANTI-Luc™ 4 Lucia/Gaussia (luciferase detection reagent), respectively. Thus, you may choose the readout depending on your laboratory equipment utilizing a spectrophotometer for SEAP or a luminometer for Lucia luciferase detection.

 

HEK-Blue-Lucia™ Null cells also feature a triple knockout (KO) of TLR3, TLR5, and TNFR. Therefore, this cell line allows for the independent study of one single TLR when used in combination with:

 

Key features:

  • Verified KO for the TLR3, TLR5, and TNFR genes
  • Functionally validated using a selection of PRR ligands and cytokines
  • Readily assessable NF-κB activation by assessing the SEAP and/or Lucia® luciferase activities

 

*Note: This cell line has been renamed. It was formerly known as "HEK-Dual™ Null". The cat. code (hkd-nullni) remains unchanged.

 

References:

1. Roebuck KA. 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res:429-38.
2. Ohta K, et al. 2014. Toll-like receptor (TLR) expression and TLR‑mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. (5):2377-82.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Tested applications

Screening of PRR agonists or inhibitors

Cell type
Epithelial
Growth properties
Adherent
Tissue origin
Human embryonic kidney cells
Reporter gene
SEAP
Lucia®
Antibiotic resistance
Blasticidin
Zeocin®
Growth medium

Complete DMEM (See TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated

CONTENTS

Contents

  • Product: 
    HEK-Blue-Lucia™ Null Cells
  • Cat code: 
    hkd-nullni
  • Quantity: 
    3-7 x 10^6 cells
Includes:

1 ml of Zeocin® (100 mg/ml) 1 ml of Normocin™ (50 mg/ml) 1 ml of QB reagent and 1 ml of QB buffer 1 tube of QUANTI-Luc™ 4 Reagent

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid Nitrogen Vapor
    Stability: 20 passages

    Caution:

    • Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.

DOCUMENTS

Documents

HEK-Blue-Lucia™ Null Cells

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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