
HEK-Blue-Lucia™ Null Cells
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Cat.code:
hkd-nullni
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ABOUT
Double NF-κB–readout HEK293 reporter cells
InvivoGen offers a human embryonic kidney 293 (HEK293)-derived cell line, specifically designed to assess the distinct role of various Toll-like receptors (TLRs):
— HEK-Blue-Lucia™ Null cells*
These cells express two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia® luciferase. Stimulation of HEK-Blue-Lucia™ Null cells with TLR agonists triggers the activation of the artificial NF-κB-inducible promoter and the subsequent production of SEAP. It also promotes the expression of Lucia® luciferase, which is knocked in (KI) downstream of the endogenous (more physiological) IL-8 promoter. IL-8 (interleukin 8) is a chemokine produced in response to TLR agonists in an NF-κB/AP-1-dependent manner [1-2].
This feature enables the double readout study of the NF-κB/AP-1 pathway, by monitoring the activity of SEAP and Lucia® luciferase using QUANTI-Blue™ Solution (SEAP detection reagent) or QUANTI-Luc™ 4 Lucia/Gaussia (luciferase detection reagent), respectively. Thus, you may choose the readout depending on your laboratory equipment utilizing a spectrophotometer for SEAP or a luminometer for Lucia luciferase detection.
HEK-Blue-Lucia™ Null cells also feature a triple knockout (KO) of TLR3, TLR5, and TNFR. Therefore, this cell line allows for the independent study of one single TLR when used in combination with:
- HEK-Blue-Lucia™ hTLR2 cells
- HEK-Blue-Lucia™ hTLR3 cells
- HEK-Blue-Lucia™ mTLR4 cells
- HEK-Blue-Lucia™ hTLR5 cells
- HEK-Blue-Lucia™ mTLR7 cells
- HEK-Blue-Lucia™ hTLR9 cells
Key features:
- Verified KO for the TLR3, TLR5, and TNFR genes
- Functionally validated using a selection of PRR ligands and cytokines
- Readily assessable NF-κB activation by assessing the SEAP and/or Lucia® luciferase activities
*Note: This cell line has been renamed. It was formerly known as "HEK-Dual™ Null". The cat. code (hkd-nullni) remains unchanged.
References:
1. Roebuck KA. 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res:429-38.
2. Ohta K, et al. 2014. Toll-like receptor (TLR) expression and TLR‑mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. (5):2377-82.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Screening of PRR agonists or inhibitors
Complete DMEM (See TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated
CONTENTS
Contents
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Product:HEK-Blue-Lucia™ Null Cells
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Cat code:hkd-nullni
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Quantity:3-7 x 10^6 cells
1 ml of Zeocin® (100 mg/ml) 1 ml of Normocin™ (50 mg/ml) 1 ml of QB reagent and 1 ml of QB buffer 1 tube of QUANTI-Luc™ 4 Reagent
Shipping & Storage
- Shipping method: Dry ice
- Liquid Nitrogen Vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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