HEK-Lucia™ Null Cells
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Cat.code:
hkl-null
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ABOUT
Lucia luciferase reporter HEK293 cells
HEK-Lucia™ Null cells are HEK293-derived cells that stably express the Lucia luciferase secreted reporter gene.
This reporter gene is under the control of the interferon (IFN)-inducible promoter which is comprised of the IFN-stimulated genes (ISG) 54 promoter enhanced by multimeric IFN-stimulated response elements (ISRE).
HEK-Lucia™ Null cells respond to human type I IFNs.
The levels of IFN-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
HEK-Lucia™ Null cells are resistant to G418, hygromycin, and Zeocin®. They should be maintained in growth medium supplemented with Zeocin®.
Note: HEK-Lucia™ Null cells endogenously express NOD1, TLR3, and TLR5.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Type I interferon (IFN) activation cellular assays
Complete DMEM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HEK-Lucia™ Null Cells
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Cat code:hkl-null
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Quantity:3-7 x 10^6 cells
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent (sufficient to prepare 25 ml)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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