HEK-Lucia™ Null Cells
HEK-Lucia™ Null Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Lucia luciferase reporter HEK293 cells |
3-7 x 10e6 cells |
hkl-null |
You may also need : QUANTI-Luc™ Gold | View more associated products ▼
Lucia luciferase reporter HEK293 cells
HEK-Lucia™ Null cells are HEK293-derived cells that stably express the Lucia luciferase secreted reporter gene.
This reporter gene is under the control of the interferon (IFN)-inducible promoter which is comprised of the IFN-stimulated genes (ISG) 54 promoter enhanced by a multimeric IFN-stimulated response elements (ISRE).
HEK-Lucia™ Null cells respond to human type I IFNs.
The levels of IFN-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.
HEK-Lucia™ Null cells are resistant to G418, hygromycin, and Zeocin®. They should be maintained in growth medium supplemented with Zeocin®.
Note: HEK-Lucia™ Null cells endogenously express NOD1, TLR3 and TLR5.
Back to the topSpecifications
Antibiotic resistance: G418, hygromycin, and Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10%heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 µg/ml)
Quality Control:
- The reporter activity has been validated by stimulating these cells with type I interferons (IFNs)
- The cell line stability for 20 passages following thawing has been verified
- HEK-Lucia™ Null cells are guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial of HEK-Lucia™ Null cells (3-7 x 106 cells)
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)
Shipped on dry ice (Europe, USA & Canada)