Spike (S) and S-derived Coding Sequences
SARS-CoV-2 Spike plasmids for cellular expression and protein production
InvivoGen offers a collection of codon-optimized plasmids for the expression of the SARS-CoV-2 Spike (S) as well as various fragments:
- Spike with the ER-retention signal removed*
- RBD (Receptor Binding Domain)
- S1 subunit
- S2 subunit
These plasmids have been specifically designed for the expression and production of the Spike protein, or fragments, for the screening of novel inhibitors of SARS-CoV-2 host entry (e.g. neutralizing antibodies) by cell-based or non-cell-based assays.
Click here for more information on the SARS-CoV-2 spike.
InvivoGen also offers:
Plasmids for mammalian cell expression
These plasmids encode the full-length Spike protein* with either a functional furin cleavage site, suitable for fusion assays, or an inactive furin cleavage site for optimized surface expression and detection by flow cytometry.
Various SARS-CoV-2 variants are available:
- Wuhan-Hu-1 | (Clade 19)
- G614 variant | (Clade 20)
- Alpha | B.1.1.7 variant | United Kingdom (Clade 20I)
- Beta | B.1.351 variant | South Africa (Clade 20H)
- Epsilon | B.1.429 variant | California (Clade 20C)
- Gamma | P.1 variant | Brazil (Clade 20J)
- Iota| B.1.526 variant | New York(Clade 20C)
More variants coming soon…
Click here for more information on the emerging SARS-CoV-2 Variants
Plasmids for expression and production of Spike protein fragments
These plasmids encode Spike fragments from the original Wuhan-Hu-1 isolate. These fragments are tagged with a C-terminal Fc or His tag to facilitate their production. Additionally, they include an exogenous signal sequence to ensure maximum protein secretion and production. The use of these plasmids for tagged protein production has been confirmed in-house.
The following Fc- or His-tagged protein fragments are available: