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CpG-free LacZ::Sh

pSELECT-zeo-LacZSh Unit size Cat. code Docs Qty Price
20 µg
psetz-laczsh
+-
$433.00

InvivoGen has engineered a CpG-free fusion gene between the lacZ and the Sh ble genes. The Sh ble gene confers Zeocin™ resistance. The lacZ gene codes for the enzyme β-galactosidase which catalyzes the hydrolysis of the substrate X-Gal to produce a blue color that is easily visualized under a microscope. The CpG-free lacZ gene is ten times more active than the wild-type gene in mammalian cells. This LacZ::Sh fusion gene enables a better and faster selection of Zeocin-resistant clones.

The synthetic LacZ::Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells.

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Specifications

Gene: CpG-free LacZ::Sh
Description: Synthetic β-Galactosidase-Zeocin resistance fusion gene
Backbone: pSELECT-zeo
Selection: Zeocin™

341 CpG in native Gene

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Contents

• 20 μg of pSELECT-zeo-LacZ::Sh plasmid provided as lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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