CpG-free LacZ::Sh

Synthetic reporter / resistance fusion gene in expression plasmid

ABOUT

Synthetic reporter / resistance fusion gene in expression plasmid

InvivoGen has engineered a CpG-free fusion gene between the lacZ and the Sh ble genes. The Sh ble gene confers Zeocin™ resistance.

The lacZ gene codes for the enzyme β-galactosidase which catalyzes the hydrolysis of the substrate X-Gal to produce a blue color that is easily visualized under a microscope.

The CpG-free lacZ gene is ten times more active than the wild-type gene in mammalian cells. This LacZ::Sh fusion gene enables a better and faster selection of Zeocin-resistant clones.

The synthetic LacZ::Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
CpG-free LacZ::Sh
Plasmid backbone
pSELECT-zeo
Antibiotic resistance
Zeocin®
Gene promoter
hEF1-HTLV prom
Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pSELECT-zeo-LacZSh
  • Cat code: 
    psetz-laczsh
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended DNA is stable more than one year at -20˚C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pSELECT-zeo-LacZSh

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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