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InvivoGen has engineered a CpG-free fusion gene between the lacZ and the Sh ble genes. The Sh ble gene confers Zeocin™ resistance. The lacZ gene codes for the enzyme β-galactosidase which catalyzes the hydrolysis of the substrate X-Gal to produce a blue color that is easily visualized under a microscope. The CpG-free lacZ gene is ten times more active than the wild-type gene in mammalian cells. This LacZ::Sh fusion gene enables a better and faster selection of Zeocin-resistant clones.
The synthetic LacZ::Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells.
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