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CpG-free Fcy::fur

pSELECT-zeo-FcyFur Unit size Cat. code Docs Qty Price
20 µg
psetz-fcyfur
+-
$433.00

Suicide genes code for enzymes, such as cytosine deaminase (CD), that convert non-toxic compounds (prodrugs) into toxic products. CD converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.

To increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase (UPRT) to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase. CD::UPRT fusions are 100 times more efficient than the CD genes alone and provide greater bystander effect. The CD::UPRT fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in mammalian and E.coli cells.

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Specifications

Gene: CpG-free Fcy::fur
Description: Synthetic S. cerevisiae cytosine deaminase-uracil phosphoribosyl transferase fusion gene
Backbone: pSELECT-zeo
Selection: Zeocin

33 CpG in native gene.

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Contents

• 20 μg of pSELECT-zeo-FcyFur plasmid provided as lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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