MDP Control

Muramyldipeptide (L-L isomer, inactive)

MDP Control, the negative control for muramyl dipeptide (MDP), is an L-L isomer that does not activate NOD2. MDP is the minimal bioactive peptidoglycan motif common to all bacteria.

MDP has been shown to be recognized by NOD2 [1, 2]. This recognition is highly stereospecific of the L-D isomer. The core structure required for recognition of NOD2 is MurNAc attached to L-Ala and D-isoGln.

Replacement of L-Ala for D-Ala (or D-isoGln for L-isoGln) eliminates the ability of muramyl dipeptide to stimulate NOD2 [2]. MDP Control contains L-Ala and L-isoGln.

References:

1. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Inohara N. et al., 2003. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn's disease.J Biol Chem. 278(8):5509-12.

Specifications

Working concentration: 10 ng - 10 µg/ml

Solubility: 10 mg/ml in water

Formula: C19H32N4O11

Molecular weight: 492.5

Synonym: N-Acetylmuramyl-L-Alanyl-L-Isoglutamine (L-L isomer)

Contents

MDP control is provided as a lyophilized powder.

• 5 mg Muramyl dipeptide (MDP) control, L-L isomer
• 1.5 ml sterile endotoxin-free water

MDP control is shipped at room temperature.

Store at -20°C. Upon resuspension, MDP control should be aliquoted and stored at -20°C.

Product is stable 6 months at -20°C when properly stored.

Avoid repeated freeze-thaw cycles.