L18-MDP

NOD2 Agonist - Muramyldipeptide with a C18 fatty acid chain

Muramyl dipeptide (MDP) is the minimal bioactive peptidoglycan motif common to all bacteria, the essential structure required for adjuvant activity in vaccines. MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1 or TLR2/6 associations [1,2].

In an attempt to enhance the protective activity against bacterial infection, numerous derivatives of MDP have been synthesized. Among them, L18-MDP, a 6-O-acyl derivative with a stearoyl fatty acid, showed the highest activity [3].

In HEK-Blue™ NOD2 cells, L18-MDP was 10 times more efficient than MDP to induce NF-κB activation.

References:

1. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Inohara N. et al., 2003. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn's disease.J Biol Chem. 278(8):5509-12.
3. Matsumoto K. et al., 1981. Stimulation of nonspecific resistance to infection induced by 6-O-acyl muramyl dipeptide analogs in mice. Infect Immun. 32(2):748-58.

Specifications

Specificity: NOD2 agonist

Working concentration: 1 - 100 ng/ml

Solubility: 1 mg/ml in water

Formula: C37H66N4O12

Molecular weight: 758.94 g/mol

Synonym: 6-O-stearoyl-N-Acetyl-muramyl-L-alanyl-D-isoglutamine

Contents

• 1 mg L18-MDP
• 1.5 ml sterile endotoxin-free water

L18-MDP is shipped at room temperature.

Store at -20°C. Upon resuspension, prepare aliquots of L18-MDP and store at -20°C.

 Resuspended product is stable for 6 months at -20°C when properly stored.

Avoid repeated freeze-thaw cycles.