Nucleocapsid (N) Expression Vector

SARS-CoV-2 Nucleocapsid coding sequence

GENE DESCRIPTION

Nucleocapsid (N) is a phosphoprotein that associates with the viral RNA genome and forms the ribonucleoprotein core [1]. N features two RNA-binding domains in N-terminal and C-terminal. Each domain supports distinct functions, including RNA chaperoning, incorporation, and packing as a helical "beads-on-a-string" conformation [1, 2]. 
pUNO1-SARS2-N contains the wild-type coding sequence of SARS-CoV-2 Nucleocapsid. (See Details and Specifications for more information).

PLASMID DESCRIPTION

pUNO1-SARS2-N features a potent mammalian expression cassette comprised of the ubiquitous human EF1α-HTLV composite promoter and the SV40 polyadenylation (pAn) signal. The ORF is flanked by unique restriction sites (AgeI and NheI) to facilitate its subcloning. The plasmid is selectable with blasticidin in both E. coli and mammalian cells. It can be used for transient or stable transfection. It contains no tag.

QUALITY CONTROL

• Fully sequenced ORF
• Predominant supercoiled conformation

Learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.

Learn more about the SARS-CoV-2 Nuclecocaspid protein.

References

1. Chang C et al., 2006. Modular organization of SARS coronavirus nucleocapsid protein. J. Biom. Sci. 13:59-72.
2. Neuman B.W. & Buchmeier M.J., 2016. Supramolecular architecture of the coronavirus particle. Advances in virus research. 96:1-27.