Nucleocapsid (N) Expression Vector

SARS-CoV-2 N gene

ABOUT

SARS-CoV-2 Nucleocapsid coding sequence

GENE DESCRIPTION

Nucleocapsid (N) is a phosphoprotein that associates with the viral RNA genome and forms the ribonucleoprotein core [1]. N features two RNA-binding domains in N-terminal and C-terminal. Each domain supports distinct functions, including RNA chaperoning, incorporation, and packing as a helical "beads-on-a-string" conformation [1, 2]. 
pUNO1-SARS2-N contains the wild-type coding sequence of SARS-CoV-2 Nucleocapsid. (See Details and Specifications for more information).

 

PLASMID DESCRIPTION

pUNO1-SARS2-N features a potent mammalian expression cassette comprised of the ubiquitous human EF1α-HTLV composite promoter and the SV40 polyadenylation (pAn) signal. The ORF is flanked by unique restriction sites (AgeI and NheI) to facilitate its subcloning. The plasmid is selectable with blasticidin in both E. coli and mammalian cells. It can be used for transient or stable transfection. It contains no tag.

 

QUALITY CONTROL

  • Fully sequenced ORF
  • Predominant supercoiled conformation

 

Learn more on SARS-CoV-2 Learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.

Learn more on SARS-CoV-2 Nuclecocaspid protein Learn more about the SARS-CoV-2 Nuclecocaspid protein.

 

References

1. Chang C et al., 2006. Modular organization of SARS coronavirus nucleocapsid protein. J. Biom. Sci. 13:59-72.
2. Neuman B.W. & Buchmeier M.J., 2016. Supramolecular architecture of the coronavirus particle. Advances in virus research. 96:1-27.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
SARS-CoV-2 Nucleocapsid
Accession sequence

NC_045512.2

ORF size
1260 bp
Plasmid backbone
pUNO
Antibiotic resistance
Blasticidin
Gene promoter
Human EF1α/HTLV hybrid promoter
Subclone start
AgeI
Subclone end
NheI
Reconstitution buffer
Sterile water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pUNO1-SARS2-N
  • Cat code: 
    puno1-cov2-n
  • Quantity: 
    20 µg
Includes:

2 x 1 ml blasticidin at 10 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Stable at least for 1 year when stored at -20°C

DOCUMENTS

Documents

pUNO1-SARS2-N

Technical Data Sheet

Plasmid Map and Sequence

Safety Data Sheet

Plasmid Sequence

Certificate of analysis

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