pUNO1-SARS2-N
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Cat.code:
puno1-cov2-n
- Documents
ABOUT
SARS-CoV-2 Nucleocapsid coding sequence
GENE DESCRIPTION
Nucleocapsid (N) is a phosphoprotein that associates with the viral RNA genome and forms the ribonucleoprotein core [1]. N features two RNA-binding domains in N-terminal and C-terminal. Each domain supports distinct functions, including RNA chaperoning, incorporation, and packing as a helical "beads-on-a-string" conformation [1, 2].
pUNO1-SARS2-N contains the wild-type coding sequence of SARS-CoV-2 Nucleocapsid. (See Details and Specifications for more information).
PLASMID DESCRIPTION
pUNO1-SARS2-N features a potent mammalian expression cassette comprised of the ubiquitous human EF1α-HTLV composite promoter and the SV40 polyadenylation (pAn) signal. The ORF is flanked by unique restriction sites (AgeI and NheI) to facilitate its subcloning. The plasmid is selectable with blasticidin in both E. coli and mammalian cells. It can be used for transient or stable transfection. It contains no tag.
QUALITY CONTROL
- Fully sequenced ORF
- Predominant supercoiled conformation
Learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.
Learn more about the SARS-CoV-2 Nuclecocaspid protein.
References
1. Chang C et al., 2006. Modular organization of SARS coronavirus nucleocapsid protein. J. Biom. Sci. 13:59-72.
2. Neuman B.W. & Buchmeier M.J., 2016. Supramolecular architecture of the coronavirus particle. Advances in virus research. 96:1-27.
All products are for research use only, and not for human or veterinary use.
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SPECIFICATIONS
Specifications
NC_045512.2
in vitro transfection
Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.
CONTENTS
Contents
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Product:pUNO1-SARS2-N
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Cat code:puno1-cov2-n
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Quantity:20 µg
2 x 1 ml blasticidin at 10 mg/ml
Shipping & Storage
- Shipping method: Room temperature
- -20°C
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Map and Sequence
Safety Data Sheet
Plasmid Sequence
Certificate of analysis
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