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pFUSE-hIgG1e13-Fc

pFUSE-hIgG1e13-Fc1 Unit size Cat. code Docs Qty Price
20 µg
pfc1-hg1e13
+-
$536.00
pFUSE-hIgG1e13-Fc2 Unit size Cat. code Docs Qty Price
20 µg
pfc2-hg1e13
+-
$536.00

pFUSE-hIgG1e13-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated I253A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

pFUSE-hIgG1e13-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

Qiao S-W, et al. (2008). Dependence of antibody-mediated presentation of antigen on FcRn. PNAS 105(27): 9337-9342.
Strohl W.R. (2009). Optimization of Fc-mediated effector functions of monoclonal antibodies. Curr Opin Biotechnol 20: 685-691.
Petkova SB, et al. (2006). Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application in humorally mediated autoimmune disease. Int Immunol 18(12):1759-69.

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Specifications

Isotype: IgG1 human
Mutation: I253A
Characteristics:
- Decreased binding to FcRn
- Decreased half-life (enhanced antibody clearance)

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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