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HEK293 CLR Reporter Cells

CLR SEAP Reporter HEK 293 Cells

InvivoGen introduces HEK-Blue™ CLR cells, a collection of  engineered HEK293  cell lines designed to provide a rapid, sensitive and reliable method to screen and validate Dectin and Mincle agonists or antagonists.

HEK-Blue™ CLR cells stably co-express the human Dectin-1b, mouse Dectin-2 or mouse Mincle gene and an NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene that can be conveniently monitored using the SEAP detection reagents QUANTI-Blue™ or HEK-Blue™ Detection.

 

Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity.  Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), the activation of NF-kB and the subsequent production of pro-inflammatory cytokines. Dectin-1 signaling has been shown to collaborate with Toll-like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor.

Dectin-2 also plays an important role in antifungal innate immunity. This receptor binds to mannose carbohydrates, such as the mannose-capped lipoarabinomannan of mycobacteria and α-mannans found in Candida albicans. Upon binding to its ligand, Dectin-2 couples with the Fc receptor gamma chain and signals through the kinase Syk and the adaptors CARD9/Bcl-10/MALT1, triggering the activation of NF-κB and the subsequent production of pro-inflammatory cytokines.

Mincle is a multi-task danger receptor that recognizes a wide variety of ligands such as damaged cells, fungus, yeast and mycobacteria. It detects trehalose-6,6-dibehenate (TDB) and heat-killed M. tuberculosis (HKMT).

InvivoGen offers several SEAP reporter cell lines that respond to CLR ligands : a macrophage derived CLR reporter cell line, and several HEK-Blue™ reporter cell lines, engineered from human HEK293, that stably express a given CLR gene

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