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Anti-hMincle-IgG

Anti-hMincle-IgG Unit size Cat. code Docs Qty Price
Human Mincle Detection and Neutralizing antibody - Monoclonal Mouse IgG2b
100 µg
mabg-hmcl
+-
$336.00

Human Mincle Detection and Neutralizing antibody - Monoclonal Mouse IgG2b

Anti-hMincle-IgG is a monoclonal mouse IgG isotype 2b antibody specific for human Mincle (hMincle).

Mincle is a C-type lectin receptor that has emerged as an important player in innate immunity. This receptor recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, some fungi, and necrotic cells [1, 2].

Anti-hMincle-IgG has been generated in mice by immunization with a plasmid coding for hMincle. This antibody was screened for flow cytometry and neutralization activity.

 

References:

1. Yamasaki S. et al., 2009. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902
2. Brown G.D., 2008. Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100.

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Specifications

Clonality: Monoclonal antibody

Applications: Neutralizing mincle-induced cellular activation. It can also be used for flow cytometry.

Specificity: Human Mincle

Isotype: Mouse IgG2b, kappa

Clone: 15H5

Formulation: 0.2 μm filtered solution in PBS with 5% saccharose

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Contents

  • 100 μg purified Anti-hMincle-IgG, provided azide-free and lyophilized

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20°C.

stability Product is stable for 6 months. Reconstituted antibody is stable 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.

Alert Avoid repeated freeze- thaw cycles.

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Description

Mincle recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, certain fungi and necrotic cells.

Exogenous ligands for Mincle include fungal α-mannose, and the mycobacterial glycolipid, trehalose-6’6’-dimycolate (TDM), also known as cord factor the immunostimulatory component of Mycobacterium tuberculosis.

Furthermore, Mincle senses damaged cells by recognizing the endogenous damage-associated molecular patterns (DAMPs). One such DAMP identified is the spliceosome-associated protein 130 (SAP130), a soluble factor released by necrotic cells.

Mincle interacts with the Fc receptor common γ-chain (FcRγ), which triggers intracellular signaling through Syk leading to CARD9-dependent NF-κB activation. Syk induces also the mobilization of intracellular calcium (Ca2+) and the activation of the calcineurin-NFAT pathway.

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