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pNiFty3-Lucia

pNiFty3-Lucia Unit size Cat. code Docs Qty Price
pNiFty3 - mIFN-β promoter - ZeocinR - Lucia
20 µg
pnf3-lc1
+-
$480.00

Inducible Lucia luciferase reporter plasmid - mIFN-β promoter - ZeocinR

pNiFty3-Lucia plasmid is composed of two key elements: the mouse interferon beta minimal promoter and a secreted luciferase (Lucias) reporter gene.

pNiFty3-SEAP plasmid is selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.

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Specifications

Minimal Promoter: mouse IFNβ promoter

Selection: Zeocin™

Reporter Gene: Lucia luciferase

These products are covered by a Limited Use License (See Terms and Conditions).

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Contents

  • pNiFty3-Lucia plasmid is provided as 20 µg lyophilized DNA with 4 pouches of Fast-Media® (2 TB and 2 Agar), containing Zeocin™.

room temperature Products are shipped at room temperature

store Stored at -20°C.

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Description

Minimal promoter

The  proximal promoters are shorter than 500   bp and contain  transcription   factor binding sites. Upon stimulation  in  293 cells,  their expression   level remains undetectable. With the   addition of  repeated TFBS, the   proximal promoters become inducible by   the  appropriate stimulus and   drive the expression of the reporter gene.

IFN-β promoter:  the mouse IFN-β minimal promoter comprises  several positive regulatory  domains that bind different cooperating  transcription factors such as  NF-kB, IRF3 and IRF7 [1].

Reporter Gene

Lucia luciferase reporter gene: Lucia luciferase is a secreted luciferase with strong bioluminescent activity
The  Lucia reporter gene is ideal for promoter activity and gene  expression  studies. Lucia is encoded by a synthetic gene derived by  genetic  engineering of copepod luciferase genes. Lucia luciferase  activity can  be detected and quantified directly in the culture medium  of  transfected cells using InvivoGen's QUANTI-Luc™ detection reagent.

 

1.   Vodjdani G. et al., 1988. Structure and characterization of a murine   chromosomal fragment containing the interferon beta gene. J Mol Biol.   204(2):221-31.

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Details

pNiFty3-Lucia map

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Citations

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