pBOOST2 saIRF7/3

Genetic adjuvants for DNA vaccines with chimeric IRF-7/IRF-3 gene | Increase both Th1 T-cell and Th2 responses

pBOOST2 saIRF7/3 plasmids express a human or murine super-activated IRF7/3 chimeric gene.

IRF-3 and IRF-7 increase both Th1 T-cell and Th2 antibody responses by transactivating different target promoters [1].

To exploit the biological features of both IRFs, a chimeric form of IRF-7 and IRF-3 was generated by combining the DNA binding specificity of IRF-7 with the strong transactivation capacity of super-activated IRF-3.

IRF-7/3 chimera provides >10-fold greater induction of IFN-α and IFN-β promoters than super-activated IRF-3 alone [2].

pBOOST2-saIRF3 plasmids express the transgene under the control of the strong and ubiquitous EF-1α/HTLV composite promoter and are selectable in E. coli with Zeocin™.

References:

1. Sasaki S. et al., 2002. Regulation of DNA-raised immune responses by cotransfected interferon regulatory factors. J Virol.76(13):6652-9.
2. Bramson JL. et al., 2003. Super-activated interferon-regulatory factors can enhance plasmid immunization. Vaccine. 21(13-14):1363-70.