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pBOOST2 saIRF-3

pBOOST2-sahIRF3 Unit size Cat. code Docs Qty Price
pBOOST2 Human mutant IRF-3
20 µg
pbst2-sahirf3
+-
$433.00
pBOOST2-samIRF3 Unit size Cat. code Docs Qty Price
pBOOST2 Murine mutant IRF-3
20 µg
pbst2-samirf3
+-
$433.00

Th1 response

pBOOST2-saIRF3 plasmids express a human or murine mutant interferon regulatory factor 3 (IRF-3) gene. IRF-3 has been shown to primarily increase Th1 T-cell responses [1]. A constitutively active form of IRF-3 was generated by creating a single point mutation of Ser396 to Asp. This super-activated IRF-3 presents a >10-fold enhanced transactivating potential over the wild-type IRF-3 for the IFN-α and IFN-β promoters [2].

pBOOST2-saIRF3 plasmids express the transgene under the control of the strong and ubiquitous EF-1α/HTLV composite promoter and are selectable in E. coli with Zeocin™.

1. Sasaki S. et al., 2002. Regulation of DNA-raised immune responses by cotransfected interferon regulatory factors. J Virol.76(13):6652-9.
2. Servant MJ. et al., 2003. Identification of the minimal phosphoacceptor site required for in vivo activation of interferon regulatory factor 3 in response to virus and double-stranded RNA. J Biol Chem. 278(11):9441-7.

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Specifications

Gene: super-activated IRF-3 gene
Species:
Human or Murine
Backbone:
 pBOOST2
Selection: Zeocin

Th1 polarization

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Contents

- 20 µg of lyophilized DNA
- 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

Product is shipped at room temperature. Store at -20°C. Plasmids are stable up to one year when properly stored.

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Details

pBOOST2 map

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