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pNiFty2 : ISG56 - ZeocinR - SEAP
pNiFty2-56K plasmid is composed of two key elements: an ISG-56K promoter (minimal promoter of the human interferon-stimulated gene ISG-56K) and a SEAP (Secreted alkaline phosphatase) reporter gene.
pNiFty2-56K plasmids are selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.
Minimal Promoter: ISG-56K promoter
Reporter Gene: SEAP
pNiFty2-56K plasmid is provided as 20 µg lyophilized DNA with 4 pouches of Fast-Media® (2 TB and 2 Agar), containing Zeocin™.
Products are shipped at room temperature
Stored at -20°C.Back to the top
The proximal promoters are shorter than 500 bp and contain transcription factor binding sites. Upon stimulation in 293 cells, their expression level remains undetectable. With the addition of repeated TFBS, the proximal promoters become inducible by the appropriate stimulus and drive the expression of the reporter gene.
ISG-56K promoter: the minimal promoter of the human interferon-stimulated gene ISG-56K contains two interferon-stimulated regulatory element (ISRE) sites and is fully inducible by type I IFNs and interferon regulatory factors (IRFs) [1, 2].
SEAP reporter gene: Secreted alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression. SEAP expression can be rapidly and readily measured in supernatants of transfected cells. SEAP levels can be evaluated qualitatively with the naked eye and quantitatively using SEAP detection media, such as HEK-Blue™ Detection system or the SEAP Reporter Assay.
1. Wathelet MG. et al., 1987. New inducers revealed by the promoter sequence analysis of two interferon-activated human genes. Eur J Biochem. 1987 Dec 1;169(2):313-21.
2. Grandvaux N, et al., 2002. Transcriptional profiling of interferon regulatory factor 3 target genes: direct involvement in the regulation of interferon-stimulated genes. J Virol. 2002 Jun;76(11):5532-9.
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