pBOOST3 mTBK1

Enhancement of DNA vaccine immunogenicity

pBOOST3-mTBK1 plasmid expresses the mouse TANK-binding kinase 1 (TBK1) gene. TBK1, a non-canonical IkB kinase, was recently shown to mediate the adjuvant effect of DNA vaccines [1].

Administration of DNA vaccines induces the production of type I interferons and inflammatory cytokines in a CpG-independent manner but in a TBK1-dependent manner.

Therefore, co-administration of a TBK1-expressing plasmid is expected to further boost DNA vaccine-induced immunogenicity.

pBOOST3-mTBK1 contains the EF-1α/HTLV composite promoter coupled to the SV40 enhancer and is selectable in E. coli with Zeocin™.

Reference:

1. Ishii KJ. et al., 2008. TANK-binding kinase-1 delineates innate and adaptive immune responses to DNA vaccines. Nature 451: 725-729.