Invivogen
Menu

codA::upp

pUNO1-codAupp Unit size Cat. code Docs Qty Price
pUNO1 bearing the bacterial codA::upp fusion gene
20 µg
puno1-codaupp
+-
$384.00

Expression-ready ORF Clones

 

E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase.

 

Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.

Back to the top

Specifications

Bacterial codA::upp (pUNO1-codAupp)

ORF size : 1935 bp
Subclone : NcoI - NheI

Back to the top

Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

Back to the top
Customer Service
& Technical Support
Shopping cart is empty