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pUNO1 bearing the bacterial codA::upp fusion gene
E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase
Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.
Bacterial codA::upp (pUNO1-codAupp)
ORF size : 1935 bp
Subclone : NcoI - NheI