codA::upp
| pUNO1-codAupp | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pUNO1 bearing the bacterial codA::upp fusion gene |
20 µg |
puno1-codaupp |
E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase
Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.
Specifications
Bacterial codA::upp (pUNO1-codAupp)
ORF size : 1935 bp
Subclone : NcoI - NheI
Contents
- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml