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CD 14

pUNO1-hCD14 Unit size Cat. code Docs Qty Price
pUNO1 bearing the human CD14 gene
20 µg
puno1-hcd14
+-
$384.00
pUNO1-hCD14s Unit size Cat. code Docs Qty Price
pUNO1 bearing the human sCD14 gene
20 µg
puno1-hcd14s
+-
$384.00
pUNO1-mCD14 Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse CD14 gene
20 µg
puno1-mcd14
+-
$384.00

Expression-ready ORF Clones

CD14 molecule.

Membrane-bound form

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor [1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].

1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity, 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem, 276(24): 21129-35.
3. Aderem A. & R.J. Ulevitch, 2000. Toll-like receptors in the induction of the innate immune response. Nature, 406(6797):782-7.

Soluble form

Many cell types do not express CD14 on the cell membrane. This is indeed the case of epithelial cells of tissues exposed to the external environment or endothelial cells. Therefore, they depend on a soluble form of CD14, found in plasma, to correctly recognize bacterial molecular patterns, such as LPS and peptidoglycan, through TLR4 and TLR2 respectively. Immunodepletion of sCD14 from serum prevents responses to LPS, and the responses are restored by addition of sCD14 [1]. Soluble CD14 can both potentiate and down-regulate responses to LPS [2,3].

 

Alias : CD14, sCD14

 

References:

1. Frey EA. et al., 1992. Soluble CD14 participates in the response of cells to lipopolysaccharide. J Exp Med. 176(6):1665-71.
2. Backhed F. et al., 2002. TLR4-dependent recognition of lipopolysaccharide by epithelial cells requires sCD14. Cell Microbiol, 4(8):493-501.
3. Kitchens RL. et al., 2001. Plasma CD14 decreases monocyte responses to LPS by transferring cell-bound LPS to plasma lipoproteins. J Clin Invest. 108(3):485-93.

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Specifications

Human CD14 (pUNO1-hCD14)

  • ORF size : 1128 bp
  • Subclone : NcoI - EcoRI

Human CD14s (pUNO1-hCD14s)

  • ORF size : 1065bp
  • Subclone : AgeI - HpaI

Mouse CD14 (pUNO1-mCD14)

  • ORF size : 1101 bp
  • Subclone : NcoI - NheI

 

Quality control:

  • Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.
  • Plasmid DNA was purified by ion exchange chromatography.
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Contents

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Store E. coli Fast-Media® at room temperature in a dry and cool place

Fast-Media® pouches are stable 2 years when stored properly.

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C

Avoid repeated freeze-thaw cycles

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Citations

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