Jurkat cells are a human immortalized T lymphocyte cell line derived from an acute T cell leukemia. Jurkat cells are used to study T cell signaling and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.
They are also widely used for the screening of the anticancer capability and mechanism of drugs.
Activation of Jurkat cells by phorbol 12-myristate 13-acetate (PMA) plus ionomycin results in the production of large amounts of interleukin-2 (IL-2).
InvivoGen provides Jurkat cells stably transfected with one or two reporter constructs designed for the study of the NFAT (nuclear factor of activated T-cells), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) or IRF (interferon regulatory factor) pathways.