HEK-Blue™ NOD2

HEK-Blue™-NOD2 cells were obtained by co-transfection of the human or murine NOD2 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells.

NOD2 (CARD15) is a member of the family of Nod-like receptors (NLRs, also known as CATERPILLER), characterized by a nucleotide-oligomerization domain (NOD) and ligand-recognizing leucine-rich repeats. NOD2 is an intracellular pattern-recognition molecules involved in the recognition of peptidoglycan (PGN). NOD2 detects specific motifs within the PGN.

NOD2 recognizes the muramyl dipeptide (MDP) structure found in almost all bacteria. It signals via the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [1]. In addition to the NF-κB pathway, NOD2 stimulation induces the activation of MAPKs [2]. CARD9 has been implicated in the selective control of NOD2-dependent p38 and JNK signaling [3].

1. Inohara N. et al., 2000. An induced proximity model for NF-kB activation in the Nod1/RICK and RIP signaling pathways. J. Biol. Chem. 275: 27823-27831.
2. Kobayashi KS. et al., 2005. Nod2-dependent regulation of innate and adaptive immunity in the intestinal tract. Science 307: 731- 734.
3. Hsu YM. et al., 2007. The adaptor protein CARD9 is required for innate immune responses to intracellular pathogens. Nat Immunol. 8(2):198-205.