NOD1 / NF-κB / SEAP reporter HEK293 cells
HEK-Blue™-NOD1 cells were obtained by co-transfection of the human or murine NOD1 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells.
NOD1 (CARD4) is a member of the family of Nod-like receptors (NLRs, also known as CATERPILLER), characterized by a nucleotide-oligomerization domain (NOD) and ligand-recognizing leucine-rich repeats. NOD1 is an intracellular pattern-recognition molecules involved in the recognition of peptidoglycan (PGN). It detects specific motifs within the PGN.
NOD1 senses the iE-DAP dipeptide which is found in PGN of all Gram-negative and certain Gram-positive bacteria . It signals via the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 . In addition to the NF-κB pathway, NOD1 stimulation induces the activation of MAPKs .
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