HEK-Blue™ NOD1

NOD1 / NF-κB / SEAP reporter HEK293 cells

HEK-Blue™-NOD1 cells were obtained by co-transfection of the human or murine NOD1 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells.

NOD1 (CARD4) is a member of the family of Nod-like receptors (NLRs, also known as CATERPILLER), characterized by a nucleotide-oligomerization domain (NOD) and ligand-recognizing leucine-rich repeats. NOD1 is an intracellular pattern-recognition molecules involved in the recognition of peptidoglycan (PGN). It detects specific motifs within the PGN.

NOD1 senses the iE-DAP dipeptide which is found in PGN of all Gram-negative and certain Gram-positive bacteria [1]. It signals via the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [2]. In addition to the NF-κB pathway, NOD1 stimulation induces the activation of MAPKs [3].

References:

1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7.
2. Inohara N. et al., 2000. An induced proximity model for NFkappaB activation in the Nod1/RICK and RIP signaling pathways. J. Biol. Chem. 275: 27823-27831.
3. Kobayashi KS. et al., 2005. Nod2-dependent regulation of innate and adaptive immunity in the intestinal tract. Science 307: 731-734.