THP1-HMGB1-Lucia™ CellsNEW

Pyroptosis and necroptosis reporter monocytes

THP1-HMGB1-Luciacells are designed to monitor necroptosis and inflammasome-mediated pyroptosis, two forms of necrotic cell death characterized by the release of the alarmin HMGB1 (High Mobility Group Box-1) upon cell membrane rupture.

Pyroptosis is a consequence of inflammasome activation.
Typically, inflammasome activation is a two-step process. A first signal (‘priming’), provided by microbial molecules such as lipopolysaccharide (LPS), induces NF-κB-dependent expression of pro-IL1β. The second signal, provided by structurally unrelated microbial molecules (e.g. Nigericin toxin) or danger signals, triggers inflammasome multimerization. This leads to caspase-1 self-activation, proteolytic maturation of IL-1β and IL-18, and cleavage of Gasdermin D (GSDMD). Subsequent GSDMD pore formation at the cell membrane elicits a rapid cell death associated with the release of IL-1β, IL-18 and HMGB1 [1,2].

Necroptosis is mainly implicated in conditions of disease or infection and is triggered by the formation of a necrosome. The necrosome is induced upon Tumor Necrosis Factor (TNF) receptor or PRR activation in absence of caspase-8 and cIAP (cellular inhibitor of apoptosis) activity. The necrosome contains receptor-interacting serine/threonine-protein kinases RIPK1 and RIPK3, and the mixed lineage kinase-like MLKL. Phosphorylated MLKL relocalizes at the plasma membrane where it drives cell lysis and release of HMGB1, most probably through pore formation [3]. 

THP1-HMGB1-Luciacells are derived from the THP-1 human monocytic cell line and stably express a HMGB1::Lucia-luciferase fusion protein. These cells respond to commonly used inflammasome inducers and necroptosis cocktail inducers. The release of the HMGB1::Lucia protein in the extracellular milieu upon pyroptosis or necroptosis can be readily measured using the QUANTI-Luc™ detection reagent.
Levels of IL-1β can be measured by Western blot, ELISA or using InvivoGen’s HEK-Blue™ IL-1β cellular assay. Alternatively, secreted IL-18 can be detected using InvivoGen’s HEK-Blue™ IL-18 cellular assay.

THP1-HMGB1-Lucia™ cells are resistant to Zeocin™.


1. Broz P. and Dixit V.M., 2016. Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol. 16:407-20.
2. Kovacs S.B. and Miao E.A., 2017. Gasdermins: effectors of pyroptosis. Trends Cell Biol. 27:673-84.
3. Grootjans S. et al., 2017. Initiation and execution mechanisms of necroptosis: an overview. Cell Death Differ. 24:1184-95.


Figures for this product

Evaluation of inflammasome mediated-pyroptosisEvaluation of necroptosis


Antibiotic resistanceZeocin™

Growth medium – RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (FBS; 30 min at 56 °C), 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml).

Quality control

• The functionality of THP1-HMGB1-Lucia™ cells was tested using inflammasome inducers, such as nigericin.
• The stability of this cell line for 20 passages following thawing has been verified.
• THP1-HMGB1-Lucia cells are guaranteed mycoplasma-free

Shipped on dry ice

This product is covered by a Limited Use Licence (see Terms and Conditions).


  • 1 vial of THP1-HMGB1-Lucia™ Cells (3-7 x 106 cells)
  • 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
  • 100 µl Zeocin™ (100 mg/ml).
  • 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium);

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


Pyroptosis/Necroptosis Pathway


THP1-HMGB1-Lucia™ CellsNEW!

Description Pyroptosis and necroptosis reporter monocytes
Cat. Codethp-gb1lc
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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