THP1-Dual™ KO-cGAS Cells

THP1-Dual KO-cGAS cells were generated from THP1-Dual cells by stable knockout of the cGAS gene. They derive from human THP-1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual and THP1-Dual KO-cGAS cells stably express two inducible secreted reporter genes: Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase). The Lucia luciferase reporter gene is under the control of an ISG54 (interferon-stimulated gene) minimal promoter in conjunction with five IFN-stimulated response elements. The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF-kB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF) and the NF-kB pathway by assessing the activity of Lucia luciferase and SEAP, respectively. Both reporter proteins are readily measurable in the cell culture supernatant when using  QUANTI‑Luc™, a Lucia™ luciferase detection reagent and  QUANTI- Blue™, a SEAP detection reagent.

THP1-Dual™ KO-cGAS and THP1-Dual™ cells can be used to study the role of cGAS by monitoring IRF-induced Lucia luciferase activity. THP1-Dual™ KO-cGAS cells are resistant to blasticidin and Zeocin™.

Figures for this product

Figure 1: IRF INDUCTION (Lucia luciferase reporter)Figure 2: NF-kB INDUCTION (SEAP reporter)


Antibiotic resistance: Zeocin™blasticidin

Growth medium:  RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality control
Biallelic cGAS knockout is verified by functional assays, PCR and DNA sequencing.
These cells are guaranteed mycoplasma-free.

Shipped on dry ice

This product is covered by a Limited Use License (See Terms and Conditions).


• 1 vial of THP1-Dual™ KO-cGAS cells (3-7 x 106 cells) in freezing medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 100 μl Zeocin™ (100 mg/ml).
• 100 μl Blasticidin (10 mg/ml).
• 1 pouch of QUANTI-Luc™.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


THP1 reporter cells are a family of cells derived from the human monocytic THP-1 cell line, which naturally expresses many pathogen recognition receptors (PRRs), including Toll-like receptors.

They respond to ligands for certain TLRs; namely, TLR2, TLR1/2, TLR2/6, TLR4, TLR5 and TLR8. These cells can also be used to study DNA sensing pathways, as they are highly responsive to PRR agonists that trigger interferon (IFN) signaling pathways.

THP1‑Dual™ cells feature two reporter genes that enable the simultaneous study of the NF-κB and IFN signaling pathways.

Cyclic GMP-AMP synthase (cGAS, cGAMP synthase) is a critical cytosolic DNA sensor that triggers innate immune responses through the production of type I interferons (IFNs) [1]. In response to cytosolic double‑stranded DNA (dsDNA), cGAS produces the cyclic dinucleotide (CDN) 2’3’-cGAMP. CDNs bind directly to STING, leading to TBK1‑IRF3-mediated activation of IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG). The most potent agonist of human STING is 2’3’-cGAMP [2,3].

cGAS Pathway


  1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121):786-91.
  2. Gao P. et et al., 2013. Cyclic [G(2’,5’)pA(3’,5’)p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
  3. Ablasser A. et al., 2013. cGAS produces a 2’-5’-linked cyclic dinucleotide second messenger that activates STING. Nature. 498(7454):380-4.


THP1-Dual KO-cGAS Cells

Description Human THP-1 Monocytes - cGAS knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
Cat. Codethpd-kocgas
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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