THP1-Blue™ NF-κB Cells

THP1-Blue™ NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.

THP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible SEAP reporter construct. As a result, THP1-Blue™ NF-κB cells allow the monitoring of NF-κB activation by determining the activity of SEAP.
The levels of NF-κB-induced SEAP in the cell culture supernatant are readily assessed with QUANTI-Blue™, a SEAP detection reagent.

THP1-Blue™ NF-κB cells are highly responsive to PRR agonists that trigger the NF-κB pathway.

Figures for this product

NF-κB response of THP1-Blue™ NF-κB cells


Antibiotic resistance: blasticidin

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Guaranteed mycoplasma-free

Shipped on dry ice


• 1 vial of THP1-Blue™ NF-κB cells (3-7 x 106 cells) in Freezing Medium.

1 ml Normocin™ (50 mg/ml).

• 100 μl Blasticidin (10 mg/ml).

1 pouch of QUANTI-Blue™

• 10e9 cells HKLM (Heat killed Listeria monocytogenes; positive control of TLR2 activity).



THP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible SEAP reporter construct. 
THP1- Blue™ NF-κB cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
As a result, THP1-Blue™ NF-κB cells allow the monitoring of NF-κB activation by determining the activity of SEAP.

Quality Control

TLR expression was determined by RT-PCR in THP1-Blue™ NF-kB cells.

All TLR mRNAs were detected. Considering the concentration of ligands used to stimulate these cells, TLR2, TLR1/2 and TLR2/6 responses are considered to be very strong. TLR4, TLR5 and TLR8 responses are robust. Responses to TLR3, TLR7 and TLR9 are hardly detectable even when high concentrations of the cognate ligands are used.
THP1-Blue™ NF-kB cells respond to NOD1 and NOD2 agonists.

THP1-Blue™ NF-kB cells are guaranteed mycoplasma-free.


Recent articles using InvivoGen THP1-Blue™ NF-κB Cells


THP1-Blue™ NF-κB Cells

Description NF-κB SEAP Reporter Monocytes
Cat. Codethp-nfkb
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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