THP1-Dual™ KO-IFNAR2 Cells

THP1-Dual KO-IFNAR2 cells were generated from THP1-Dual cells by stable knockout of the IFNAR2 gene. They derive from human THP‑1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual and THP1-Dual KO-IFNAR2 cells stably express two inducible secreted reporter genes: Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase). The Lucia luciferase reporter gene is under the control of an ISG54 (interferon-stimulated gene) minimal promoter in conjunction with five IFN-stimulated response elements. The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF‑kB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF) pathway, by assessing the activity of Lucia luciferase and the NF-kB pathway, by monitoring the activity of SEAP. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI‑Luc™, a Lucia™ luciferase detection reagent and QUANTI- Blue™, a SEAP detection reagent.

THP1-DualKO-IFNAR2 and THP1-Dualcells can be used to study the role of IFNAR2 by monitoring IRF-induced Lucia luciferase activity. THP1-Dual KO-IFNAR2 cells are resistant to blasticidin and Zeocin™ 

Figures for this product

Figure 1: IRF INDUCTION (Lucia luciferase reporter)Figure 2: NF-kB INDUCTION (SEAP reporter)


Antibiotic resistance: Zeocin™blasticidin

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality control
Biallelic IFNAR2 knockout is verified by functional assays, PCR and DNA sequencing.
These cells are guaranteed mycoplasma-free.

Shipped on dry ice

This product is covered by a Limited Use License (See Terms and Conditions).


• 1 vial of THP1-Dual™ KO-IFNAR2 cells (3-7 x 106 cells) in freezing medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 100 μl Zeocin™ (100 mg/ml).
• 100 μl Blasticidin (10 mg/ml).
• 1 pouch of QUANTI-Luc™.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


THP1 reporter cells are a family of cells derived from the human monocytic THP-1 cell line, which naturally expresses many pathogen recognition receptors (PRRs), including Toll-like receptors.

They respond to ligands for certain TLRs; namely, TLR2, TLR1/2, TLR2/6, TLR4, TLR5 and TLR8. These cells can also be used to study DNA sensing pathways, as they are highly responsive to PRR agonists that trigger interferon (IFN) signaling pathways.

THP1‑Dual™ cells feature two reporter genes that enable the simultaneous study of the NF-κB and IFN signaling pathways.

Type I interferons (IFNs), in particular IFN-α and IFN-β, are vital for host resistance to viral infections. Their activity is mediated by the IFN-α receptor (IFNAR), which consists of two chains: IFNAR1 and IFNAR2. Following their production, these cytokines bind IFNAR and recruit the Janus kinases (JAK1 and TyK2), leading to the phosphorylation of STAT1 and STAT2, which then dimerize. These dimers interact with IFN regulatory factor 9 (IRF9), forming a complex named ISGF3, which binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression. Knockout of the IFNAR2 gene abolishes the cellular response to type I IFNs [1,2].

IFNAR2 Pathway


  1. Zao W. et al., 2008. A conserved IFN-alpha receptor tyrosine motif directs the biological response to type I IFNs. J Immunol. 180(8):5483-9.
  2. de Weerd NA. et al., 2007. Type I interferon receptors: biochemistry and biological functions. J Biol Chem. 282(28):20053-7. 


THP1-Dual KO-IFNAR2 Cells

Description Human THP-1 Monocytes - IFNAR2 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
Cat. Codethpd-koifnar2
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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