THP1-Dual™ KO-IFI16 Cells

IFI16 knockout dual reporter monocytes

THP1-Dual™ KO-IFI16 cells were generated from THP1-Dual™ cells by stable knockout of the IFI16 (Interferon-γ-inducible protein 16) gene. IFI16 is an innate immune sensor with multiple functions; DNA sensor, regulator of interferon (IFN) expression, activator of the inflammasome, and regulator of genome function [1,2]. These cell lines were derived from human THP‑1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual™ and THP1-Dual™ KO-IFI16 cells stably express two inducible secreted reporter genes: IFN-inducible Lucia luciferase and NF‑κB-inducible SEAP (secreted embryonic alkaline phosphatase). They can be used to study the role of IFI16 by monitoring IRF-induced Lucia luciferase activity, using QUANTI‑Luc™, a Lucia™ luciferase detection reagent.

THP1-Dual™ KO-IFI16 cells are resistant to the selectable markers blasticidin and Zeocin™.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)


Antibiotic resistance: Zeocin™blasticidin

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality control
Biallelic IFI16 knockout was verified by functional assays, PCR and DNA sequencing.
These cells are guaranteed mycoplasma-free.

Shipped on dry ice

This product is covered by a Limited Use License (See Terms and Conditions).


• 1 vial of THP1-Dual™ KO-IFI16 cells (3-7 x 106 cells) in freezing medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 100 μl Zeocin™ (100 mg/ml).
• 100 μl Blasticidin (10 mg/ml).
• 1 pouch of QUANTI-Luc™.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


THP1-Dual™ and THP1-Dual™ KO-IFI16 cells stably express two inducible secreted reporter genes: Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase). The Lucia luciferase reporter gene is under the control of an ISG54 (interferon-stimulated gene) minimal promoter in conjunction with five IFN-stimulated response elements. The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF‑κB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF) pathway, by assessing the activity of Lucia luciferase and the NF-κB pathway, by monitoring the activity of SEAP. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI‑Luc™, a Lucia™ luciferase detection reagent and QUANTI-Blue™, a SEAP detection reagent.


1. Jakobsen MR. & Paludan SR., 2014. IFI16: At the interphase between innate DNA sensing and genome regulation. Cytokine Growth Factor Rev. 25(6):649-55.

2. Unterholzner L. et al., 2010. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 11(11):997-1004.


Recent articles using InvivoGen THP1-Dual™ KO-IFI16 Cells


THP1-Dual KO-IFI16 Cells

Description Human THP-1 Monocytes - IFI16 knockout NF-κB-SEAP and IRF-Lucia Reporter Cells
Cat. Codethpd-koifi16
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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