THP1-Null cells

Positive control cell line for inflammasome studies

THP1-Null cells are derived from THP-1 human monocytic cells, that represent the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC and pro-caspase-1.

THP1-Null cells produce IL-1β upon stimulation with inflammasome inducers, such as MSU crystals and ATP.
As THP1-Null cells are fully efficient for NLRP3 and ASC activities, they are the positive control cell line for THP1-defASC and THP1-defNLRP3, that are deficient in ASC and NLRP3 respectively.

Figures for this product

IL-1β production in THP1-null, THP1-defASC and THP1-defNLRP3 cellsQuantitative RT-PCR analysis


Antibiotic resistance: hygromycin B

Growth Medium:  RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

 Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Hygromycin B Gold (100 mg/ml)

1 ml Normocin™ (50 mg/ml)


Quality control

The functionality of THP1-Null cells was tested using inflammasome inducers, such as MSU crystals and ATP.


THP1-Null cells are designed to study the signals involved in inflammasome activation. To become susceptible to inflammasome inducers, these cells must be induced by stimuli commonly used for induction in model systems, such as lipopolysaccharide (LPS) and phorbol 12-myristate acetate (PMA). Stimulation by LPS or differentiation with PMA induces the production of pro-IL-1β, the immature form of IL-1β. Subsequent stimulation with inflammasome inducers, such as ATP and alum crystals, leads to caspase-1 activation and IL-1β maturation and secretion. Mature IL-1β can be detected by Western blot, ELISA, or a cell-based assay.

InvivoGen has developed a new method to detect bioactive IL-1β, based on HEK293 cells specifically engineered to selectively respond to IL-1β, named HEK-Blue™ IL-1β. These cells feature the SEAP (secreted embryonic alkaline phosphotase) reporter gene under the control of an NF-kB-inducible promoter. They naturally express the IL-1β receptor (IL-1R), and all the proteins involved in the MyD88-dependent IL-1R signaling pathway that leads to NF-kB activation. Thus upon IL-1β binding to IL-1R, a signaling cascade is initiated triggering NF-kB activation and the subsequent production of SEAP. Detection of SEAP in the supernatant of HEK-Blue™ IL-1β cells can be readily assessed using QUANTI-Blue™, a SEAP detection medium. QUANTI-Blue™ turns blue in the presence of SEAP which can be easily quantified using a spectrophotometer.


Recent articles using InvivoGen THP1-Null cells



Description Positive control cell line for inflammasome studies
Cat. Codethp-null
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "".