THP1-NLRC4 cells

NLRC4 inflammasome test cell line

THP1-NLRC4 cells are derived from the THP1 human monocytic cell line, which represents the most commonly used model cell line to study inflammasome activation.

THP1-NLRC4 cells stably overexpress NLRC4 and naturally express TLR5. Stimulation of these cells with flagellin triggers TLR5 signaling leading to NF-κB activation and the production of pro-IL1β. Once in the cytosol, flagellin induces the formation of the NLRC4 inflammasome resulting in the activation of caspase-1 and the release of IL-1β.

Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line.

Figures for this product

Detection of flagellin-induced IL-1β using HEK-Blue™KD-TLR5 cells.


Antibiotic resistance: Blasticidin

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml)

Guaranteed mycoplasma-free

Shipped on dry ice


• 1 vial of THP1-NLRC4 cells (3-7 x 10e6 cells) in Freezing Medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 100 μl Blasticidin (10 mg/ml).


Quality control

Expression of NLRC4 was confirmed by qRT-PCR and a functionality assay using inflammasome inducers.

THP1-NLRC4 cells are guaranteed mycoplasma-free.


THP1-NLRC4 cells are designed to study the activation of the NLRC4 inflammasome.
Mature IL-1β can be detected by Western blot, ELISA, or a cell-based assay.
InvivoGen has developed HEK-Blue™ KD-TLR5 cells, a reporter cell line designed to monitor bioactive IL-1β secreted by THP-1 cells upon flagellin-induced NLRC4 activation.
HEK-Blue™ KD-TLR5 cells are derived from the HEK293 cell line, which endogenously expresses TLR5 and the IL-1β receptor (IL-1R). This cell line features an NF-kB-inducible SEAP reporter gene and was engineered to knock-down the expression of TLR5 to avoid activation of NF-kB upon flagellin-induced TLR5 stimulation. The knockdown of TLR5 permits the analysis of flagellin specifically for its NLRC4 stimulating activity. Binding of IL-1β to IL-1R initiates a signaling cascade leading to the activation of NF-kB and the subsequent production of SEAP.
Detection of SEAP in the supernatant of HEK-Blue™ KD-TLR5 cells can be readily assessed using the QUANTI-Blue™ assay. Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line.
THP1-NLRC4 and HEK-Blue™ KD-TLR5 cell lines can be used sequentially (option 1) or co-cultured to save time (option 2).

THP-1/HEK-Blue™ IL-1β Assay :

Detection of IL-1beta in THP-1 supernatants



THP1-NLRC4 cells

Description NLRC4 inflammasome test cell line
Cat. Codethp-nlrc4
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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