THP1-ASC-GFP Cells

Real-time monitoring of ASC-dependent inflammasomes 

THP1-ASC-GFP cells are inflammasome reporter cells that allow the monitoring of ASC (apoptosis-associated speck-like protein with a CARD)-dependent inflammasome formation using fluorescence microscopy.

These cells are derived from the THP-1 human monocytic cell line and stably express a gene encoding an ASC::GFP fusion protein for which expression is driven by an NF-κB-inducible promoter. Hence, in resting cells, no GFP signal is detected. Upon the first step of inflammasome activation (‘priming”), NF-kB-dependent ASC::GFP expression is induced and can be observed throughout the cytoplasm. Following the second step of inflammasome activation, ASC::GFP polymerizes to form a macromolecular, micrometer-sized complex. The number of ASC::GFP positive cells and localization of fluorescent ASC specks can be determined by using time-lapse confocal or high-resolution fluorescence microscopy. In most cells only one speck forms upon inflammasome activation [1,2].

THP1-ASC-GFP cells are resistant to Zeocin™.

Figures for this product

Activation of THP1-ASC-GFP CellsVisualization of ASC speck formation by fluorescence microscopy

Specifications

Antibiotic resistance: Zeocin

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml)

Quality control: The functionality of THP1 ASC-GFP cells has been tested using inflammasome inducers, such as the microbial toxin nigericin and transfected poly(dA:dT). The stability of this cell line for 20 passages following thawing has been verified. THP1 ASC-GFP cells are guaranteed mycoplasma-free.

Shipped on dry ice.

This product is covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial of THP1 ASC-GFP cells (3-7 x 106 cells) in Freezing Medium.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. 
• 100 μl Zeocin (100 mg/ml).

Ref.

1. Hoss F. et al., 2017. Assembly and regulation of ASC specks. Cell. Mol. Life Sci. 74:1211-29.
2. Stutz A. et al., 2013. ASC speck formation as a readout for inflammasome activation. Methods Mol Biol. 1040:91-101.

ORDERING

THP1-ASC-GFP

Description Human THP-1 Monocytes - ASC speck reporter cells
Cat. Codethp-ascgfp
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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