HEK-Blue™ IL-1β Cells

IL-1β Sensor cells

HEK-Blue™ IL-1β cells allow to detect bioactive IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They derive from HEK-Blue™ TNF-α/IL-1β cells in which the TNF-α response has been blocked. Therefore, HEK-Blue™ IL-1β cells respond specifically to IL-1. They express a NF-κB/AP-1-inducible SEAP reporter gene. Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1β cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.

HEK-Blue™ IL-1β cells are useful to monitor IL-1β in inflammasome activation studies. These cells detect the presence of IL-1β in samples which triggers the production of SEAP. Levels of SEAP can be easily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-1β cells with recombinant human IL-1β can be blocked by the neutralizing monoclonal anti-hIL-1β-IgG antibody.

Figures for this product

IL-1β response following inflammasome activationResponse of HEK-Blue™ IL-1β cells to IL-1β and TNF-αCytokine response profile of HEK-Blue™ IL-1β cellsNeutralization of IL-1β response

Specifications

Antibiotic resistance: hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice

Detects human and murine IL-1β (α)
• Detection range for human IL-1β: 100 pg - 100 ng/ml
• Detection range for murine IL-1β: 10 ng - 1 µg/ml

Contents

• 1 vial containing 3-5 x 106 cells

• 100 μl Hygromycin B Gold (100 mg/ml)

• 100 μl Zeocin™ (100 mg/ml)

• 1 ml Normocin™ (50 mg/ml)

• 1 pouch of QUANTI-Blue™

Description

HEK-Blue IL1-beta pathway

Interleukin-1β (IL-1β) is an important mediator of the inflammatory response to infection and injury. IL-1β binds to the type 1 IL-1 receptor (IL-1R) which requires the IL-1 receptor accessory protein (IL-1RAcP) to transduce a signal. IL-1β signaling is mediated by MyD88, IRAK-1/2 and TRAF-6 leading to the activation of NF-κB and AP-1.

Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1β cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.

 

THP-1/HEK-Blue™ IL-1β Assay

THP-1/HEK-Blue IL-1β Assay

1- Production of IL-1β by THP-1 cells: Typically, THP-1 cells are pretreated with phorbol 12-myristate acetate (PMA) to become more susceptible to inflammasome activators, then are primed with lipopolysaccharide (LPS). These treatments induce the production of pro-IL-1β, the immature form of IL-1β.
Subsequent stimulation with inflammasome inducers, such as crystals or ATP, leads to NRLP3 and caspase-1 activation resulting in IL-1β maturation and secretion.

2- Detection of IL-1β by HEK-Blue™ IL-1β cells: IL-1β-containing THP-1 supernatants are added to HEK-Blue™ IL-1β cells leading to NF-κB activation and the subsequent production of SEAP. The presence of SEAP in HEK-Blue™ IL-1β supernatants is assessed using QUANTI-Blue™, a SEAP detection medium.

Citations

Recent articles using InvivoGen HEK-Blue™ IL-1β Cells

ORDERING

HEK-Blue™ IL-1β cells

Cat. Codehkb-il1b
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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