HEK-Blue™ IL-18 Cells

IL-18 Sensor Cells

HEK-Blue™ IL-18 cells are designed to detect bioactive IL-18 by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK293 derived cells with the genes encoding IL-18R and IL-18RAP. In addition, the TNF-α and the IL-1β responses have been blocked. Therefore, HEK-Blue™ IL-18 cells respond specifically to IL-18. These cells express an NF-κB/AP-1-inducible SEAP reporter gene.

Binding of IL-18 to the heterodimeric IL-18 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.

Figures for this product

Response of HEK-Blue™ IL-18 cells to human and mouse IL-18


Antibiotic resistance: blasticidin, hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice

Specificity: Detects human & murine IL-18

Detection range: 10 pg - 1 ng/ml for human IL-18, 3 ng - 100 ng/ml for mouse IL-18.



• 1 vial containing 3-7 x 106 cells.

• 2 x 1 ml HEK-Blue™ Selection (250X concentrate); a solution containing several selection antibiotics.

• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.

• 1 pouch of QUANTI-Blue™ (SEAP detection medium).


HEK-Blue IL18 pathway

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that causes a wide variety of biological effects associated with infection, inflammation and autoimmune processes. It binds to an heterodimeric receptor consisting of IL-18R and IL-18 receptor accessory protein (IL18RAP) which then induces signaling pathways that involve MyD88, and TRAF-6 leading to the activation of NF-κB and AP-1.


Recent articles using InvivoGen HEK-Blue™ IL-18 Cells


HEK-Blue™ IL-18 cells

Cat. Codehkb-hmil18
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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