HEK-Blue™ IFN-α/β Cells

Human Type I IFN Sensor Cells

HEK-Blue™ IFN-α/β cells allow the detection of bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT2 and IRF9 genes to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2 and STAT1) are naturally expressed in sufficient amounts. The cells were further transfected with a SEAP reporter gene under the control of the IFN-α/β inducible ISG54 promoter. Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α can be blocked by anti-hIFN-α-IgA, a neutralizing monoclonal antibody.

Figures for this product

Response of HEK-Blue™ IFN-α/β cells to IFNCytokine response profile of HEK-Blue™ IFN-α/βcellsNeutralization of IFN-α response


Antibiotic resistance: blasticidin, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice

Detects human type I interferons
• Detection range for human IFN-α: 1 - 104 IU/ml
• Detection range for human IFN-β: 10 - 104 IU/ml


1 vial containing 3-7 x 106 cells

100 μl Blasticidin (10 mg/ml)

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (SEAP detection medium)


HEK-Blue IFN-alpha/beta cells pathway

Type I interferons, in particular interferon alpha (IFN-α) and interferon beta (IFN-β), play a vital role in host resistance to viral infections. They signal mainly through the JAK-STAT pathway. Following their production, IFN-α and IFN-β bind to a common receptor (IFNAR) and recruit the Janus kinases (JAK1 and TyK2).
JAKs phosphorylate STAT1 and STAT2, which then dimerize and interact with IFN regulatory factor 9 (IRF9), forming a complex named ISGF3. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.
Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP.


HEK-Blue™ IFN-α/β cells

Cat. Codehkb-ifnab
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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