HEK-Blue™ IL-33 Cells

IL-33 Sensor Cells

HEK-Blue™ IL-33 cells are designed to detect bioactive IL-33 by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK293-derived cells with the human IL1RL1 gene. In addition, the TNF-α and the IL-1β responses have been blocked. Therefore, HEK-Blue™ IL-33 cells respond specifically to IL-33. These cells express an NF-κB/AP-1- inducible SEAP reporter gene.

Binding of human IL-33 to the heterodimeric IL-33 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.


Antibiotic resistance: blasticidin, hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice

Specificity: Detects human IL-33

Detection range: 0.5 - 100 ng/ml


• 1 vial containing 3-7 x 106 cells.

• 2 x 1 ml HEK-Blue™ Selection (250X concentrate); a solution containing several selection antibiotics.

• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.

• 1 pouch of QUANTI-Blue™ (SEAP detection medium).


HEK-Blue IL-33/IL-1beta cells pathway

Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation and inflammation. IL-33 mediates its biological effects through IL1RL1 (also known as ST2), a receptor expressed on Th2 and mast cells.

IL-33 and IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex. IL-33 signaling leads to the activation of NF-kB and MAP kinases, and the production of Th2-associated cytokines.


Recent articles using InvivoGen HEK-Blue™ IL-33 Cells


HEK-Blue™ IL-33 cells

Cat. Codehkb-hil33
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".